Yamakuni T, Araki K, Takahashi Y
FEBS Lett. 1985 Aug 19;188(1):127-30. doi: 10.1016/0014-5793(85)80888-7.
Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two-dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-free system containing rat cerebellar mRNA. The developmental changes of mRNA-dependent protein biosynthesis were also examined. During postnatal 10-30 days, a rapid increase of mRNA levels for spot 35 protein was observed. The application of the new 45Ca-binding assay procedure revealed that this protein is a Ca-binding protein.
