Pochet R, Parmentier M, Lawson D E, Pasteels J L
Brain Res. 1985 Oct 21;345(2):251-6. doi: 10.1016/0006-8993(85)91000-5.
Two proteins from rat brain reacting against anti-chick intestinal vitamin D-dependent calcium-binding protein were characterized in terms of their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and their molecular size. The proteins were present in the isolated cytoplasm and were produced following translation of brain mRNA in the rabbit reticulocyte lysate system. Their apparent molecular weight was 29,000 and 27,000 daltons whereas rat kidney contained only one protein cross-reacting with this antiserum and with a molecular weight of 27,000 daltons.
针对抗鸡肠维生素D依赖性钙结合蛋白产生反应的两种来自大鼠脑的蛋白质，根据它们在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上的迁移率和分子大小进行了表征。这些蛋白质存在于分离出的细胞质中，并且是在兔网织红细胞裂解物系统中由脑信使核糖核酸翻译产生的。它们的表观分子量分别为29,000和27,000道尔顿，而大鼠肾脏仅含有一种与该抗血清发生交叉反应且分子量为27,000道尔顿的蛋白质。
