Yoo Seungyeop, Lee Hyun Jong
School of Chemical, Biological and Battery Engineering, Gachon University, Seongnam-si, Republic of Korea.
Cells Tissues Organs. 2025;214(2):128-147. doi: 10.1159/000541416. Epub 2024 Sep 12.
Despite significant advances in three-dimensional (3D) cell culture technologies, creating accurate in vitro models that faithfully recapitulate complex in vivo environments remains a major challenge in biomedical research. Traditional culture methods often fail to simultaneously facilitate critical cell-cell and cell-extracellular matrix (ECM) interactions while providing control over mechanical and biochemical properties.
This review introduces the spheroid-hydrogel-integrated biomimetic system (SHIBS), a groundbreaking approach that synergistically combines spheroid culture with tailored hydrogel technologies. SHIBS uniquely bridges the gap between traditional culture methods and physiological conditions by offering unprecedented control over both cellular interactions and environmental properties. We explore how SHIBS is revolutionizing fields ranging from drug discovery and disease modeling to regenerative medicine and basic biological research. The review discusses current challenges in SHIBS technology, including reproducibility, scalability, and high-resolution imaging, and outlines ongoing research addressing these issues. Furthermore, we envision the future evolution of SHIBS into more sophisticated organoid-hydrogel-integrated biomimetic systems and its integration with cutting-edge technologies such as microfluidics, 3D bioprinting, and artificial intelligence.
SHIBS represents a paradigm shift in 3D cell culture technology, offering a unique solution to recreate complex in vivo environments. Its potential to accelerate the development of personalized therapies across various biomedical fields is significant. While challenges persist, the ongoing advancements in SHIBS technology promise to overcome current limitations, paving the way for more accurate and reliable in vitro models. The future integration of SHIBS with emerging technologies may revolutionize biomimetic modeling, potentially reducing the need for animal testing and expediting drug discovery processes. This comprehensive review provides researchers and clinicians with a holistic understanding of SHIBS technology, its current capabilities, and its future prospects in advancing biomedical research and therapeutic innovations.
Despite significant advances in three-dimensional (3D) cell culture technologies, creating accurate in vitro models that faithfully recapitulate complex in vivo environments remains a major challenge in biomedical research. Traditional culture methods often fail to simultaneously facilitate critical cell-cell and cell-extracellular matrix (ECM) interactions while providing control over mechanical and biochemical properties.
This review introduces the spheroid-hydrogel-integrated biomimetic system (SHIBS), a groundbreaking approach that synergistically combines spheroid culture with tailored hydrogel technologies. SHIBS uniquely bridges the gap between traditional culture methods and physiological conditions by offering unprecedented control over both cellular interactions and environmental properties. We explore how SHIBS is revolutionizing fields ranging from drug discovery and disease modeling to regenerative medicine and basic biological research. The review discusses current challenges in SHIBS technology, including reproducibility, scalability, and high-resolution imaging, and outlines ongoing research addressing these issues. Furthermore, we envision the future evolution of SHIBS into more sophisticated organoid-hydrogel-integrated biomimetic systems and its integration with cutting-edge technologies such as microfluidics, 3D bioprinting, and artificial intelligence.
SHIBS represents a paradigm shift in 3D cell culture technology, offering a unique solution to recreate complex in vivo environments. Its potential to accelerate the development of personalized therapies across various biomedical fields is significant. While challenges persist, the ongoing advancements in SHIBS technology promise to overcome current limitations, paving the way for more accurate and reliable in vitro models. The future integration of SHIBS with emerging technologies may revolutionize biomimetic modeling, potentially reducing the need for animal testing and expediting drug discovery processes. This comprehensive review provides researchers and clinicians with a holistic understanding of SHIBS technology, its current capabilities, and its future prospects in advancing biomedical research and therapeutic innovations.
尽管三维（3D）细胞培养技术取得了重大进展，但创建能够忠实地重现复杂体内环境的精确体外模型仍然是生物医学研究中的一项重大挑战。传统培养方法往往无法在控制机械和生化特性的同时，促进关键的细胞间和细胞与细胞外基质（ECM）的相互作用。
本综述介绍了球体-水凝胶集成仿生系统（SHIBS），这是一种开创性的方法，将球体培养与定制水凝胶技术协同结合。SHIBS通过对细胞相互作用和环境特性提供前所未有的控制，独特地弥合了传统培养方法与生理条件之间的差距。我们探讨了SHIBS如何正在彻底改变从药物发现、疾病建模到再生医学和基础生物学研究等各个领域。该综述讨论了SHIBS技术当前面临的挑战，包括可重复性、可扩展性和高分辨率成像，并概述了针对这些问题正在进行的研究。此外，我们设想了SHIBS未来向更复杂的类器官-水凝胶集成仿生系统的发展，以及它与微流控、3D生物打印和人工智能等前沿技术的整合。
SHIBS代表了3D细胞培养技术的范式转变，为重现复杂的体内环境提供了独特的解决方案。它在加速各个生物医学领域个性化疗法开发方面的潜力巨大。尽管挑战依然存在，但SHIBS技术的不断进步有望克服当前的局限性，为更准确、可靠的体外模型铺平道路。SHIBS与新兴技术的未来整合可能会彻底改变仿生建模，有可能减少动物试验的需求并加快药物发现过程。这篇全面的综述为研究人员和临床医生提供了对SHIBS技术、其当前能力以及在推进生物医学研究和治疗创新方面的未来前景的全面理解。
尽管三维（3D）细胞培养技术取得了重大进展，但创建能够忠实地重现复杂体内环境的精确体外模型仍然是生物医学研究中的一项重大挑战。传统培养方法往往无法在控制机械和生化特性的同时，促进关键的细胞间和细胞与细胞外基质（ECM）的相互作用。
本综述介绍了球体-水凝胶集成仿生系统（SHIBS），这是一种开创性的方法，将球体培养与定制水凝胶技术协同结合。SHIBS通过对细胞相互作用和环境特性提供前所未有的控制，独特地弥合了传统培养方法与生理条件之间的差距。我们探讨了SHIBS如何正在彻底改变从药物发现、疾病建模到再生医学和基础生物学研究等各个领域。该综述讨论了SHIBS技术当前面临的挑战，包括可重复性、可扩展性和高分辨率成像，并概述了针对这些问题正在进行的研究。此外，我们设想了SHIBS未来向更复杂的类器官-水凝胶集成仿生系统的发展，以及它与微流控、3D生物打印和人工智能等前沿技术的整合。
SHIBS代表了3D细胞培养技术的范式转变，为重现复杂的体内环境提供了独特的解决方案。它在加速各个生物医学领域个性化疗法开发方面的潜力巨大。尽管挑战依然存在，但SHIBS技术的不断进步有望克服当前的局限性，为更准确、可靠的体外模型铺平道路。SHIBS与新兴技术的未来整合可能会彻底改变仿生建模，有可能减少动物试验的需求并加快药物发现过程。这篇全面的综述为研究人员和临床医生提供了对SHIBS技术、其当前能力以及在推进生物医学研究和治疗创新方面的未来前景的全面理解。
