Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China.
Int J Biol Macromol. 2024 Nov;279(Pt 4):135557. doi: 10.1016/j.ijbiomac.2024.135557. Epub 2024 Sep 13.
Enzyme immobilization is an effective strategy for achieving efficient and sustainable enzyme catalysis. As a kind of promising enzyme-loading materials, the systematic research on zirconium based metal organic frameworks (Zr-MOFs) about immobilization performance at molecular level is still in its initial stage. In this work, UiO-66 was functionalized with various groups (-H, -NH, -COOH, -OH, -2OH) for the immobilization of cytochrome c (Cyt c) and antioxidant enzyme catalase (CAT). Then the effects of surface-functionalized UiO-66 derivatives on the loading efficiency, enzyme stability and catalysis kinetics were systematically investigated. In addition, the affinity constants of Cyt c and CAT towards UiO-66-series MOFs carriers were also compared. The results have shown that hydroxyl group functionalized UiO-66 represents the highest enzyme loading capacity, enhanced activity and improved stability for Cyt c and CAT possibly due to high surface area and suitable microenvironments as well as enhanced affinity towards the enzymes provided by the introduction of a single hydroxyl group. Our research would foresee immense potential of MOFs in engineering biocatalysts.
酶固定化是实现高效和可持续酶催化的有效策略。作为一种有前途的酶负载材料，锆基金属有机框架（Zr-MOFs）在分子水平上的固定化性能的系统研究仍处于起步阶段。在这项工作中，UiO-66 用各种基团（-H、-NH、-COOH、-OH、-2OH）进行功能化，用于细胞色素 c（Cyt c）和抗氧化酶过氧化氢酶（CAT）的固定化。然后系统研究了表面功能化 UiO-66 衍生物对负载效率、酶稳定性和催化动力学的影响。此外，还比较了 Cyt c 和 CAT 与 UiO-66 系列 MOFs 载体的亲和常数。结果表明，羟基功能化的 UiO-66 对 Cyt c 和 CAT 表现出最高的酶负载能力、增强的活性和提高的稳定性，这可能是由于引入单个羟基基团提供的高表面积和合适的微环境以及对酶的增强亲和力。我们的研究将预见 MOFs 在工程生物催化剂方面的巨大潜力。
