Ye Niru, Kou Xiaoxue, Shen Jun, Huang Siming, Chen Guosheng, Ouyang Gangfeng
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
Department of Radiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China.
Chembiochem. 2020 Sep 14;21(18):2585-2590. doi: 10.1002/cbic.202000095. Epub 2020 May 19.
Metal-organic frameworks (MOFs) with attractive properties such as high surface area, tunable porosity, designable functionality and excellent stability, have aroused great interest from researchers as the matrices for enzyme immobilization. Recently, several efficient strategies including surface immobilization, post-synthetic infiltration and in situ encapsulation have been explored. MOF-immobilized enzymes, named enzymes@MOFs, show remarkably enhanced stability and recyclability, accelerating cell-free biocatalysis in diverse applications. This concept will impart the typical strategies for enzyme immobilization with MOFs, and their potential applications.
金属有机框架材料（MOFs）具有诸如高比表面积、可调孔隙率、可设计功能和出色稳定性等吸引人的特性，作为酶固定化的基质引起了研究人员的极大兴趣。最近，人们探索了几种有效的策略，包括表面固定化、合成后渗透和原位封装。固定在MOF上的酶，即酶@MOF，表现出显著增强的稳定性和可循环性，在各种应用中加速了无细胞生物催化。本文将阐述用MOF进行酶固定化的典型策略及其潜在应用。
