Pourshahrestani Sara, Unalan Irem, Zeimaran Ehsan, Xu Zhiyan, Roether Judith A, Kerpes Andrea, Janko Christina, Alexiou Christoph, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
Institute of Polymeric Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.
Bioact Mater. 2025 Aug 12;54:71-85. doi: 10.1016/j.bioactmat.2025.07.046. eCollection 2025 Dec.
Polyphenols such as tannic acid (TA) with antibacterial and antioxidant activities have recently attracted significant attention for wound healing applications. Mesoporous bioactive glass nanoparticles (MBGNs) have also garnered considerable interest to be employed as nanocarriers of therapeutic biomolecules. This study focuses on the fabrication of TA-loaded MBGNs which were doped with two well-known biologically active elements, copper (Cu) and zinc (Zn). The effect of TA loading on the antioxidant and biological properties of the nanoparticles was investigated in the context of potential wound healing applications. As proven with various techniques, TA was successfully loaded on CuMBGNs and ZnMBGNs. With increasing TA concentration, the phenolic content in the nanoparticles was found to increase and CuMBGNs-TA and ZnMBGNs-TA were found to possess 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The nanoparticles not only showed biocompatibility towards normal human dermal fibroblast (NHDF) cells, but they were also found to be hemocompatible. In comparison to CuMBGNs-TA leachates resulting in wound closure rate of ∼66 %-∼83 %, the dissolution products of ZnMBGNs-TA led to higher wound closure rate (>90 %). Our results demonstrate that CuMBGNs or ZnMBGNs are suitable nanocarriers for antioxidant TA and are candidates to promote wound healing.
诸如具有抗菌和抗氧化活性的单宁酸（TA）等多酚类物质，最近在伤口愈合应用方面引起了广泛关注。介孔生物活性玻璃纳米颗粒（MBGNs）作为治疗性生物分子的纳米载体也备受关注。本研究聚焦于制备负载TA的MBGNs，其中掺杂了两种著名的生物活性元素铜（Cu）和锌（Zn）。在潜在的伤口愈合应用背景下，研究了TA负载量对纳米颗粒抗氧化和生物学性能的影响。通过各种技术证明，TA成功负载在CuMBGNs和ZnMBGNs上。随着TA浓度的增加，纳米颗粒中的酚类含量增加，并且发现CuMBGNs-TA和ZnMBGNs-TA具有1,1-二苯基-2-苦基肼（DPPH）自由基清除活性。这些纳米颗粒不仅对正常人皮肤成纤维细胞（NHDF）表现出生物相容性，而且还被发现具有血液相容性。与CuMBGNs-TA浸出液导致的伤口闭合率约为66% - 83%相比，ZnMBGNs-TA的溶解产物导致更高的伤口闭合率（>90%）。我们的结果表明，CuMBGNs或ZnMBGNs是抗氧化剂TA的合适纳米载体，并且是促进伤口愈合的候选者。
