The Jackson Laboratory, Bar Harbor, ME, USA.
School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA, USA.
Sci Data. 2024 Sep 12;11(1):996. doi: 10.1038/s41597-024-03839-3.
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family of cytokines and has been found to have anti-inflammatory and pro-inflammatory properties in various cellular and disease contexts. OSM signals through two receptor complexes, one of which includes OSMRβ. Here, we investigated OSM-OSMRβ signaling in adult mouse hematopoietic stem cells (HSCs) using the conditional Osmr mouse model B6;129-Osmr/J. We crossed Osmr mice to interferon-inducible Mx1-Cre, which is robustly induced in adult HSCs. From these mice, we isolated HSCs by flow cytometry, stimulated with recombinant OSM or vehicle for 1 hour, and assessed gene expression changes in control versus Osmr knockout HSCs by RNA-seq. This data may be utilized to investigate OSMRβ -dependent and -independent OSM signaling as well as the transcriptional effects of an IL-6 family cytokine on mouse HSCs to further define its anti-inflammatory versus pro-inflammatory properties.
抑瘤素 M（OSM）是白细胞介素-6（IL-6）家族细胞因子的一员，在各种细胞和疾病环境中被发现具有抗炎和促炎特性。OSM 通过两种受体复合物发出信号，其中一种复合物包括 OSMRβ。在这里，我们使用条件性 Osmr 小鼠模型 B6;129-Osmr/J 研究了成年小鼠造血干细胞（HSCs）中的 OSM-OSMRβ 信号传导。我们将 Osmr 小鼠与干扰素诱导的 Mx1-Cre 杂交，该基因在成年 HSCs 中被强烈诱导。从这些小鼠中，我们通过流式细胞术分离 HSCs，用重组 OSM 或载体刺激 1 小时，然后通过 RNA-seq 比较对照和 Osmr 敲除 HSCs 中的基因表达变化。这些数据可用于研究 OSMRβ 依赖性和非依赖性 OSM 信号传导以及细胞因子对小鼠 HSCs 的转录效应，以进一步定义其抗炎与促炎特性。
