Department of Organismal Biology-Systematic Biology, Science for Life Laboratory, Evolutionary Biology Centre, Uppsala University, 75236, Uppsala, Sweden.
Population Ecology Group, Institute of Ecology and Evolution, Friedrich Schiller University Jena, 07743, Jena, Germany.
Sci Data. 2024 Sep 12;11(1):997. doi: 10.1038/s41597-024-03858-0.
Morabine grasshoppers in the Vandiemenella viatica species group, which show karyotype diversity, have been studied for their ecological distribution and speciation in relation to their genetic and chromosomal diversity. They are good models for studying sex chromosome evolution as "old" and newly emerged sex chromosomes co-exist within the group. Here we present a reference genome for the viatica19 chromosomal race, that possesses the ancestral karyotype within the group. Using PacBio HiFi and Hi-C sequencing, we generated a chromosome-level assembly of 4.09 Gb in span, scaffold N50 of 429 Mb, and complete BUSCO score of 98.1%, containing 10 pseudo-chromosomes. We provide Illumina datasets of males and females, used to identify the X chromosome. The assembly contains 19,034 predicted protein-coding genes, and a total of 75.21% of repetitive DNA sequences. By leveraging HiFi reads, we mapped the genome-wide distribution of methylated bases (5mC and 6 mA). This comprehensive assembly offers a robust reference for morabine grasshoppers and supports further research into speciation and sex chromosome diversification within the group and its related species.
我们为 Vandiemenella viatica 种组中的 Morabine 蚱蜢提供了参考基因组，该基因组具有种组内的祖先核型。我们使用 PacBio HiFi 和 Hi-C 测序技术，生成了一个跨度为 4.09Gb 的染色体水平组装，支架 N50 为 429Mb，完整的 BUSCO 评分达到 98.1%，包含 10 个假染色体。我们提供了雄性和雌性的 Illumina 数据集，用于识别 X 染色体。组装包含 19,034 个预测的蛋白质编码基因，总重复 DNA 序列为 75.21%。通过利用 HiFi 读取，我们绘制了全基因组甲基化碱基（5mC 和 6mA）的分布图谱。这个全面的组装为 Morabine 蚱蜢提供了一个强大的参考，支持进一步研究种组内和相关物种的物种形成和性染色体多样化。
