State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China.
Sci Data. 2024 Sep 19;11(1):1029. doi: 10.1038/s41597-024-03870-4.
The Nilaparvata muiri (Hemiptera: Delphacidae) is a sibling species of a destructive rice insect pest, the brown planthopper (BPH), Nilaparvata lugens. Here, we generated a high-quality chromosome-level genome assembly of N. muiri using a combination of the PacBio HiFi sequencing, Illumina short-read sequencing and Hi-C scaffolding technologies. The genome assembly (524.9 Mb) is anchored to 15 pseudochromosomes, with a scaffold N50 of 43.3 Mb and 99.1% BUSCO completeness. It contains 188.1 Mb repeat sequences and 13204 protein-coding genes. As a closely related species within the same genus as the significant pest, N. lugens, the chromosome-level genome assembly of N. muiri will provide important support for the better analysis of pathogenicity mechanisms of N. lugens based on comparative genomics.
黑尾叶蝉（Hemiptera：飞虱科）是一种具有破坏性的水稻害虫——褐飞虱（BPH）的近缘种。在这里，我们使用 PacBio HiFi 测序、Illumina 短读测序和 Hi-C 支架技术组合，生成了黑尾叶蝉的高质量染色体水平基因组组装。基因组组装（524.9Mb）被锚定到 15 个假染色体上，支架 N50 为 43.3Mb，99.1%的 BUSCO 完整性。它包含 188.1Mb 的重复序列和 13204 个蛋白质编码基因。作为与重要害虫褐飞虱同属的近缘种，黑尾叶蝉的染色体水平基因组组装将为基于比较基因组学更好地分析褐飞虱的致病性机制提供重要支持。
