Agricultural Botany Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Biochemistry Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Sci Rep. 2024 Sep 13;14(1):21373. doi: 10.1038/s41598-024-71165-0.
Salinity stress negatively affects the growth and yield of crops worldwide. Onion (Allium cepa L.) is moderately sensitive to salinity. Beneficial microorganisms can potentially confer salinity tolerance. This study investigated the effects of endomycorrhizal fungi (M), Pseudomonas putida (Ps) and their combination (MPs) on onion growth under control (0 ppm), moderate (2000 ppm) and high (4000 ppm) NaCl salinity levels. A pot experiment was conducted with sandy loam soil and onion cultivar Giza 20. Results showed that salinity reduced growth attributes, leaf pigments, biomass and bulb yield while increasing oxidative stress markers. However, individual or combined inoculations significantly increased plant height, bulb diameter and biomass production compared to uninoculated plants under saline conditions. MPs treatment provided the highest stimulation, followed by Pseudomonas and mycorrhizae alone. Overall, dual microbial inoculation showed synergistic interaction, conferring maximum benefits for onion growth, bulbing through integrated physiological and biochemical processes under salinity. Bulb yield showed 3.5, 36 and 83% increase over control at 0, 2000 and 4000 ppm salinity, respectively. In conclusion, combined application of mycorrhizal-Pseudomonas inoculations (MPs) effectively mitigate salinity stress. This approach serves as a promising biotechnology for ensuring sustainable onion productivity under saline conditions.
盐胁迫会对全球范围内的作物生长和产量产生负面影响。洋葱（Allium cepa L.）对盐度中度敏感。有益微生物可能具有耐盐性。本研究探讨了内生真菌（M）、假单胞菌（Ps）及其组合（MPs）在对照（0 ppm）、中度（2000 ppm）和高（4000 ppm）NaCl 盐度下对洋葱生长的影响。在砂壤土和洋葱品种 Giza 20 中进行了盆栽实验。结果表明，盐度降低了生长属性、叶片色素、生物量和鳞茎产量，同时增加了氧化应激标志物。然而，与未接种植物相比，单独或组合接种在盐胁迫条件下显著增加了株高、鳞茎直径和生物量的产生。MPs 处理提供了最高的刺激，其次是假单胞菌和单独的菌根。总的来说，双重微生物接种表现出协同作用，通过整合生理和生化过程，为洋葱生长、鳞茎发育提供了最大的益处。与对照相比，在 0、2000 和 4000 ppm 盐度下，鳞茎产量分别增加了 3.5、36 和 83%。总之，联合应用菌根-假单胞菌接种（MPs）可以有效缓解盐胁迫。这种方法是在盐胁迫条件下确保洋葱可持续生产力的一种有前途的生物技术。
