State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Nat Commun. 2019 Jan 11;10(1):141. doi: 10.1038/s41467-018-08083-z.
Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that the RABBIT EARS and HANABA TARANU transcription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals.
基因调控网络通过特定基因的表达来控制发育。在种子植物中，位于茎尖分生组织（SAM）中的自我更新干细胞从 SAM 周围区域产生叶子。起始后，叶子形成极性模式以形成平面形状。在这里，我们比较了 SAM 和叶域之间的翻译 RNA。使用翻译核糖体亲和纯化和 RNA 测序来量化目标域中的基因表达，我们生成了一个特定于域的翻译组图谱，涵盖了代表性的营养阶段 SAM 和叶域。我们讨论了这些域的预测细胞功能，并提供了证据表明，表面上似乎不相关的域利用了共同的调节模块。实验后续表明，RABBIT EARS 和 HANABA TARANU 转录因子在腋芽分生组织起始中起作用。该数据集为进一步研究芽发育以及对内部和环境信号的反应提供了一个社区资源。
