National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov of Ministry of Health of Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2024 Aug;177(4):497-501. doi: 10.1007/s10517-024-06214-9. Epub 2024 Sep 12.
The morphometric characteristics of brain tissue were studied based on autopsy material from 49 deceased newborns divided into 7 groups based on the time after death. Samples were taken from the upper (frontal lobe) and lower (occipital lobe) regions relative to the supine position of the body. Paraffin sections were prepared from these samples and stained with hematoxylin and eosin. Histological preparations were analyzed using an image analysis system to determine the area of gliocyte nuclei, cross-cut blood vessels, and expanded area around them in the white matter of the brain. Based on these data, severity indices were calculated for both cellular (pericellular) and vascular (perivascular) expansion. The dynamics of changes in morphometric parameters within the range of cellular and vascular alterations on brain tissue specimens, which reflect the development of postmortem hypostasis and autolysis in this organ, can be used to estimate the duration of the postmortem interval. At the same time, these changes, when combined with other non-specific postmortem findings, should be distinguished from long-term pathological processes and diseases that may have occurred during life.
基于 49 例死亡新生儿的尸检材料，根据死后时间将其分为 7 组，研究脑组织的形态计量学特征。从相对于身体仰卧位的上部（额叶）和下部（枕叶）区域采集样本。从这些样本中制备石蜡切片，并使用苏木精和曙红染色。使用图像分析系统分析组织学制剂，以确定脑白质中神经胶质细胞核、交叉切割血管以及其周围扩展区域的面积。基于这些数据，为细胞（细胞周）和血管（血管周）扩张计算严重程度指数。在反映该器官死后凝固和自溶发展的组织标本中形态计量参数变化的范围内，可以使用这些参数来估计死后间隔时间。同时，这些变化与其他非特异性死后发现相结合，应与可能在生前发生的长期病理过程和疾病区分开来。
