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从生物质中高效一步提取短杆菌肽S的工艺

Efficient one step extraction process of Gramicidin S from biomass.

作者信息

Lvova Ksenia, Vecino Xanel, Pérez-Cid Benita, Moldes Ana B, Cruz José M

机构信息

Chemical Engineering Department, School of Industrial Engineering-CINTECX, University of Vigo, Campus As Lagoas-Marcosende, Vigo, Spain.

Department of Analytical and Food Chemistry, Faculty of Chemistry-CINTECX, University of Vigo, Campus As Lagoas-Marcosende, Vigo, Spain.

出版信息

Front Bioeng Biotechnol. 2024 Aug 29;12:1452796. doi: 10.3389/fbioe.2024.1452796. eCollection 2024.

DOI:10.3389/fbioe.2024.1452796
PMID:39267904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11390548/
Abstract

Currently, Gramicidin S (GR-S) is produced enzymatically with the drawback of the presence of trifluoroacetic acid (TFA) or produced by fermentation involving several separation and purification steps. Therefore, this study is focused on the use of green solvents as unique extraction step to produce Gramicidin S from microbial biomass of Among the tested solvents, such as ethanol, acidic ethanol or buffer phosphate, the most favorable was acidic ethanol, extracting 96% of Gramicidin S from cells with a purity of 90%. Using acidic ethanol, extraction time within the range of 30-120 min exhibited minimal impact on Gramicidin S yield, whereas the biomass-to-extractant ratio emerged as a critical parameter. Gramicidin S extracts were characterized using Fourier Transform Infrared Spectroscopy (FTIR), Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS), and Electrospray Ionization Mass Spectrometry (ESI-MS) coupled with Ultra Performance Liquid Chromatography (UPLC) and compared with commercial Gramicidin S.

摘要

目前，短杆菌肽S（GR - S）是通过酶法生产的，但存在三氟乙酸（TFA）残留的缺点，或者是通过涉及多个分离和纯化步骤的发酵法生产。因此，本研究聚焦于使用绿色溶剂作为从微生物生物质中生产短杆菌肽S的独特提取步骤。在测试的溶剂中，如乙醇、酸性乙醇或磷酸盐缓冲液，最有利的是酸性乙醇，其从细胞中提取出96%的短杆菌肽S，纯度为90%。使用酸性乙醇时，30 - 120分钟范围内的提取时间对短杆菌肽S产量的影响最小，而生物质与萃取剂的比例则是一个关键参数。短杆菌肽S提取物使用傅里叶变换红外光谱（FTIR）、基质辅助激光解吸/电离飞行时间质谱（MALDI - TOF - MS）以及与超高效液相色谱（UPLC）联用的电喷雾电离质谱（ESI - MS）进行表征，并与市售短杆菌肽S进行比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/e2400f273bad/fbioe-12-1452796-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/5e1cc86cdcd7/fbioe-12-1452796-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/398e7ca1f485/fbioe-12-1452796-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/bf0829bb0317/fbioe-12-1452796-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/0306855b9638/fbioe-12-1452796-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/e2400f273bad/fbioe-12-1452796-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/5e1cc86cdcd7/fbioe-12-1452796-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/398e7ca1f485/fbioe-12-1452796-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/bf0829bb0317/fbioe-12-1452796-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/0306855b9638/fbioe-12-1452796-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/194d/11390548/e2400f273bad/fbioe-12-1452796-g005.jpg

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