Krishnakumar Sharanya, Khalid Abdul Azeez Mohamed, Sowndarya Jothipandian, Krishnasamy Lakshmi, Nithyanand Paramasivam
Department of Microbiology, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamilnadu, India.
Biofilm Biology Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA Deemed University, Tirumalaisamudram, Thanjavur, Tamil Nadu, India.
Iran J Microbiol. 2024 Aug;16(4):443-449. doi: 10.18502/ijm.v16i4.16302.
Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.
This study was conducted over a period of 1 year, where 296 isolates of were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.
A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.
Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics.
尽管抗菌药物不断发展，但葡萄球菌感染仍是全球主要的传染病之一。了解耐甲氧西林金黄色葡萄球菌（MRSA）感染的局部模式和流行情况对于治疗起着关键作用。本研究的目的是通过表型和基因型方法鉴定MRSA菌株，并分析来自一家三级护理医院患者的MRSA菌株的抗生素敏感性模式。
本研究为期1年，从各种临床标本中分离出296株菌株。采用改良的 Kirby Bauer 纸片扩散法检测分离菌株的抗生素敏感性。通过头孢西丁纸片扩散试验检测耐甲氧西林情况。
共发现104株为MRSA，192株为甲氧西林敏感金黄色葡萄球菌（MSSA）。在104株MRSA分离株中，对10株多重耐药菌株进行了16S rRNA基因测序分析。所有10株菌株与导致某些严重生物膜介导的临床表现（如囊性纤维化和器械介导感染）的菌株有99%的匹配度。使用结晶紫染色对生物膜进行定量，并使用扫描电子显微镜分析其产生生物膜的能力，并与基因库进行匹配。
因此，这些系统发育分析有助于治疗患者并对抗抗生素耐药性。
