Kushida K, Ishizaki T
J Chromatogr. 1985 Feb 27;338(1):131-9. doi: 10.1016/0378-4347(85)80077-3.
We describe a method for the simultaneous determination of valproic acid with four other antiepileptic drugs (phenobarbital, phenytoin, carbamazepine, and primidone) in plasma by high-performance liquid chromatography. These drugs are extracted from plasma by adding a small volume of acetonitrile following saturation with ammonium sulphate. An aliquot of the extract is then injected on a reversed-phase column with a methanol-water mobile phase. The total time required for the whole analytical process including the plasma pretreatment and chromatography is approximately 20 min. The assay method is simple, rapid, reproducible and specific, and considered, therefore, suitable for both emergency and routine uses in monitoring these antiepileptic drugs simultaneously.
我们描述了一种通过高效液相色谱法同时测定血浆中丙戊酸及其他四种抗癫痫药物（苯巴比妥、苯妥英、卡马西平和扑米酮）的方法。在血浆用硫酸铵饱和后加入少量乙腈，从血浆中提取这些药物。然后将一份提取物注入以甲醇 - 水为流动相的反相柱中。整个分析过程（包括血浆预处理和色谱分析）所需的总时间约为20分钟。该测定方法简单、快速、可重现且具有特异性，因此被认为适用于同时监测这些抗癫痫药物的急诊和常规应用。
