Martínez de Muñoz D, Arenas R, Chávez González O
Department of Physiology, Biophysics and Neurosciences, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, D.F., Mexico.
J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):377-83. doi: 10.1016/0378-4347(95)00554-4.
A method for the simultaneous determination of HEPP (D,L-3-hydroxy-3-ethyl-3-phenylpropionamide), a member of a new homologous series of phenylamide-derivative anticonvulsants, with six other antiepileptic drugs (ethosuximide, primidone, phenobarbital, phenytoin, carbamazepine and clonazepam) in plasma by high-performance liquid chromatography is described. These drugs are extracted from plasma by adding an equal volume of acetonitrile. An aliquot of the extract is then injected on a reversed-phase column with a acetonitrile-methanol-phosphate buffer mobile phase. The total time required for the whole analytical process, including the plasma pretreatment and chromatography, is approximately 30 min. The assay method is simple, rapid and reproducible, and therefore considered suitable for routine use in clinical investigations monitoring HEPP simultaneously with common antiepileptic drugs.
本文描述了一种用高效液相色谱法同时测定血浆中新型苯基酰胺衍生物抗惊厥药同系物HEPP（D，L-3-羟基-3-乙基-3-苯基丙酰胺）以及其他六种抗癫痫药物（乙琥胺、扑米酮、苯巴比妥、苯妥英、卡马西平和氯硝西泮）的方法。通过加入等体积的乙腈从血浆中提取这些药物。然后取一份提取物注入到以乙腈-甲醇-磷酸盐缓冲液为流动相的反相柱上。整个分析过程所需的总时间，包括血浆预处理和色谱分析，约为30分钟。该测定方法简单、快速且可重复，因此被认为适用于在临床研究中同时监测HEPP和常用抗癫痫药物的常规使用。
