He Yunxiao, Zhang Yujiao, Li Jiangnan, Ren Zhiyi, Zhang Wenjing, Zuo Xianghua, Zhao Wei, Xing Ming, You Jian, Chen Xia
National and Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, School of Life Sciences, Jilin University, Changchun, Jilin, China.
Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences, Yanbian, Jilin, China.
Front Plant Sci. 2024 Aug 29;15:1412416. doi: 10.3389/fpls.2024.1412416. eCollection 2024.
Plants adapt to cold stress through a tightly regulated process involving metabolic reprogramming and tissue remodeling to enhance tolerance within a short timeframe. However, the precise differences and interconnections among various organs during cold adaptation remain poorly understood. This study employed dynamic transcriptomic and metabolite quantitative analyses to investigate cold adaptation and subsequent de-adaptation in , a species known for its robust resistance to abiotic stress. Our findings revealed distinct expression patterns in most differentially expressed genes (DEGs) encoding transcription factors and components of the calcium signal transduction pathway within the two organs under cold stress. Notably, the long-distance transport of carbon sources from source organs (leaves) to sink organs (roots) experienced disruption followed by resumption, while nitrogen transport from roots to leaves, primarily in the form of amino acids, exhibited acceleration. These contrasting transport patterns likely contribute to the observed differences in cold response between the two organs. The transcriptomic analysis further indicated that leaves exhibited increased respiration, accumulated anti-stress compounds, and initiated the ICE-CBF-COR signaling pathway earlier than roots. Differential expression of genes associated with cell wall biosynthesis suggests that leaves may undergo cell wall thickening while roots may experience thinning. Moreover, a marked difference was observed in phenylalanine metabolism between the two organs, with leaves favoring lignin production and roots favoring flavonoid synthesis. Additionally, our findings suggest that the circadian rhythm is crucial in integrating temperature fluctuations with the plant's internal rhythms during cold stress and subsequent recovery. Collectively, these results shed light on the coordinated response of different plant organs during cold adaptation, highlighting the importance of inter-organ communication for successful stress tolerance.
植物通过一个严格调控的过程来适应冷胁迫，该过程涉及代谢重编程和组织重塑，以在短时间内增强耐受性。然而，在冷适应过程中，各个器官之间的确切差异和相互联系仍知之甚少。本研究采用动态转录组学和代谢物定量分析方法，对一种以其对非生物胁迫具有强大抗性而闻名的物种在冷适应及随后的去适应过程进行了研究。我们的研究结果揭示了在冷胁迫下，两个器官中大多数编码转录因子和钙信号转导途径成分的差异表达基因（DEG）具有不同的表达模式。值得注意的是，碳源从源器官（叶片）到库器官（根）的长距离运输经历了中断，随后恢复，而从根到叶的氮运输，主要以氨基酸的形式，表现出加速。这些相反的运输模式可能导致了两个器官在冷响应中观察到的差异。转录组分析进一步表明，叶片比根表现出更高的呼吸作用、积累了抗逆化合物，并更早启动了ICE-CBF-COR信号通路。与细胞壁生物合成相关基因的差异表达表明，叶片可能会经历细胞壁增厚，而根可能会变薄。此外，在两个器官的苯丙氨酸代谢中观察到显著差异，叶片有利于木质素的产生，而根有利于黄酮类化合物的合成。此外，我们的研究结果表明，昼夜节律在冷胁迫及随后的恢复过程中，将温度波动与植物内部节律整合方面至关重要。总的来说，这些结果揭示了不同植物器官在冷适应过程中的协同反应，突出了器官间通讯对于成功耐受胁迫的重要性。
