Joseph Olajumoke Olufunmilayo, Dahunsi Samuel Olatunde, Okoh Anthony
Microbiology Programme, College of Agriculture, Engineering, and Sciences, Bowen University Iwo, Osun State Nigeria.
The Radcliffe Institute for Advanced Study, Harvard University, Cambridge, MA, USA.
New Microbes New Infect. 2024 Aug 25;62:101468. doi: 10.1016/j.nmni.2024.101468. eCollection 2024 Dec.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that is responsible for COVID-19 pandemic, is a zoonotic RNA virus that has been reported in animals, including domestic animals. Due to the growing concern of health threat that could arise from active transmission of SARS-CoV-2 between pet owners and their pets, there is need to monitoring the emergence of a highly pathogenic strain of SARS-CoV-2 that is capable of transboundary infection, or a serious outbreak among human populations.
We carried out a search in English, on PubMed and NCBI (National Center for Biotechnology Information) SARS-CoV-2 resources for relevant journals and nucleotide sequence data, that were published between 2019 and 2023. The CoVsurver mutations application on GISAID webpage was used to analyse mutation, nucleotide sequence alignment was carried out using MAFFT (Multiple Alignment using Fast Fourier Transform) version 7 and maximum likelihood tree was constructed by bootstrapping with 1000 replicates on MEGA 11 software.
A total of 47 mutations at the Spike gene region were identified, and mutation D614 was the most observed mutation. Nucleotide sequences of isolates from domestic animals had high sequence identity with Wuhan-Hu-1 reference sequence and the representative sequences of previously circulating VOCs from humans.
This reveals that there is spill over of previously circulating variants of concern (VOC) to household pets from their infected owners. Hence, there is an urgent need for more intense surveillance to be carried out globally to monitor evolution of SARS-CoV-2 coronaviruses as a result of human - pet association.
导致新冠疫情的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种人畜共患的RNA病毒，已在包括家畜在内的动物中被报道。由于人们越来越担心SARS-CoV-2在宠物主人与其宠物之间的主动传播可能带来的健康威胁，因此有必要监测能够进行跨界感染的高致病性SARS-CoV-2毒株的出现，或在人群中发生的严重疫情。
我们在PubMed和NCBI（美国国立生物技术信息中心）的SARS-CoV-2资源中用英文搜索了2019年至2023年期间发表的相关期刊和核苷酸序列数据。使用GISAID网页上的CoVsurver突变应用程序分析突变，使用MAFFT（基于快速傅里叶变换的多序列比对）7版本进行核苷酸序列比对，并在MEGA 11软件上通过1000次重复自展构建最大似然树。
在刺突基因区域共鉴定出47个突变，其中D614突变是最常见的突变。家畜分离株的核苷酸序列与武汉-Hu-1参考序列以及人类先前流行的VOC的代表性序列具有高度的序列同一性。
这表明先前流行的关注变异株（VOC）从受感染的主人传播到家养宠物身上。因此，迫切需要在全球范围内加强监测，以监测由于人宠关联导致的SARS-CoV-2冠状病毒的进化。
