Spinola S M, Cannon J G
J Immunol Methods. 1985 Jul 16;81(1):161-5. doi: 10.1016/0022-1759(85)90132-2.
We compared bovine serum albumin, commercial non-fat dry milk, and Tween 20 as blocking agents for immunologic probing of bacterial proteins transferred to nitrocellulose sheets. There were quantitative and qualitative differences in antigens detected that depended on which blocking agents were used. We suggest that several methods for blocking and washing nitrocellulose should be compared when Western blotting is used to detect immunologically reactive proteins.
