Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Tokyo General Hospital, Tokyo, Japan.
Microbiol Spectr. 2024 Oct 3;12(10):e0135424. doi: 10.1128/spectrum.01354-24. Epub 2024 Sep 13.
Reports of novel species of α-hemolytic have increased recently. However, limited information exists regarding the pathogenicity of these species, with the exception of and . In this study, a quinolone-resistant α- strain, MTG105, was isolated from the sputum of a patient with pneumonia. This strain was first identified as at the hospital laboratory; however, it exhibited unique genetic features upon further analysis. Digital DNA-DNA hybridization and average nucleotide identity based on BLAST values from whole-genome sequencing revealed MTG105 to be a novel species closely related to . Although MTG105 carried two copies of the pneumolysin gene, similar to , this isolate exhibited susceptibility to optochin under both aerobic and 5% CO conditions. Notably, no biochemical features could be used to definitively identify this species. In an infection assay using organotypic lung tissue models, MTG105 induced epithelial damage comparable to that of and , possibly suggesting its potential as a pathogenic α-. The natural transformation abilities of species facilitate their exchange of genes within the same genus, resulting in the existence of species with increasingly more diverse genome structures. Therefore, the identification of this species highlights the importance of monitoring the emergence of novel species exhibiting virulence and/or multidrug resistance. This isolate was proposed as a novel species, designated sp. nov. The type strain was MTG 105 (= JCM 35953 = CCUG 76894).
The genus encompasses a wide range of bacteria with more than 60 species. Recently, there has been a notable increase in reports of novel species of α- based on genomic analysis data. However, limited information exists regarding the pathogenicity of these species. In this study, a quinolone-resistant α-hemolytic strain, MTG105, was isolated from a patient with pneumonia. Genetic analysis revealed that this species was a novel species closely related to . In an infection assay using organotypic lung tissue models, MTG105 induced epithelial damage comparable to that caused by and , strongly suggesting its potential as a pathogenic α-. The natural transformation abilities of species facilitate gene exchange within the same genus, leading to the emergence of species with increasingly diverse genome structures. Therefore, the identification of this species underscores the importance of monitoring the emergence of novel species exhibiting virulence and/or multidrug resistance.
最近有报道称新型甲型溶血性 不断出现。然而，除了 和 以外，这些物种的致病性信息有限。在这项研究中，从肺炎患者的痰中分离出一株耐喹诺酮的甲型溶血性 菌株 MTG105。该菌株最初在医院实验室被鉴定为 ，但进一步分析显示其具有独特的遗传特征。数字 DNA-DNA 杂交和全基因组测序的 BLAST 值平均核苷酸同一性表明，MTG105 是一种与 密切相关的新型物种。尽管 MTG105 携带两个肺炎球菌溶血素基因，但与 相似，该分离株在有氧和 5% CO 条件下均对奥普托欣敏感。值得注意的是，没有生化特征可用于明确鉴定该物种。在使用器官型肺组织模型进行的感染实验中，MTG105 诱导的上皮损伤与 和 相当，这可能表明其作为潜在致病性甲型的可能性。 物种的自然转化能力促进了它们在同一属内基因的交换，导致具有越来越多样化基因组结构的物种的存在。因此，该物种的鉴定凸显了监测具有毒力和/或多药耐药性的新型物种出现的重要性。该分离株被提议为一种新型物种，命名为 sp. nov. 。模式株为 MTG 105（= JCM 35953 = CCUG 76894）。
属包含 60 多种细菌，具有广泛的范围。最近，基于基因组分析数据，有报道称新型甲型不断出现。然而，这些物种的致病性信息有限。在这项研究中，从肺炎患者中分离出一株耐喹诺酮的甲型溶血性 菌株 MTG105。遗传分析表明，该物种是一种与 密切相关的新型物种。在使用器官型肺组织模型进行的感染实验中，MTG105 诱导的上皮损伤与 和 相当，强烈表明其作为潜在致病性甲型的可能性。 物种的自然转化能力促进了它们在同一属内基因的交换，导致具有越来越多样化基因组结构的物种的出现。因此，该物种的鉴定凸显了监测具有毒力和/或多药耐药性的新型物种出现的重要性。
