Moor R M, Osborn J C, Crosby I M
J Reprod Fertil. 1985 May;74(1):167-72. doi: 10.1530/jrf.0.0740167.
The development of preovulatory follicles involves an initial phase of somatic cell differentiation and a final phase, initiated by the LH surge, when both the somatic and germinal compartments alter. Abnormalities in this pattern of compartmental development after superovulation have been identified by examining follicles from control, PMSG- and FSH-treated sheep. The pattern of proteins synthesized by oocytes from untreated sheep did not differ after culture of follicles in hormone-free medium from that of germinal vesicle oocytes in vivo. Similarly, 93.5% of oocytes from sheep injected with a pituitary gonadotrophin (FSH-P) synthesized the unchanged germinal vesicle pattern of proteins during culture in an hormonally neutral culture environment. By contrast, the administration of the placental gonadotrophin, PMSG, induced in 28% of oocytes changes in the pattern of synthesis which are normally associated with maturation. An examination of follicular steroidogenesis showed that both total output and particularly oestrogen secretion was over twice as high in follicles from PMSG-treated as compared with FSH-treated animals (P less than 0.01). We conclude that the compartmental pattern of development and steroidogenesis is grossly perturbed in many follicles from PMSG-treated animals. Premature activation of the germinal compartment results in aged or abnormal oocytes and a hostile reproductive tract.
排卵前卵泡的发育包括体细胞分化的初始阶段和由促黄体生成素（LH）峰启动的最终阶段，此时体细胞和生殖细胞区室都会发生变化。通过检查来自对照、孕马血清促性腺激素（PMSG）和促卵泡素（FSH）处理的绵羊的卵泡，已确定了超排卵后这种区室发育模式的异常情况。未处理绵羊的卵母细胞合成的蛋白质模式，在无激素培养基中培养卵泡后，与体内生发泡期卵母细胞的模式没有差异。同样，在激素中性的培养环境中培养时，注射垂体促性腺激素（FSH-P）的绵羊中93.5%的卵母细胞合成了未改变的生发泡期蛋白质模式。相比之下，注射胎盘促性腺激素PMSG会使28%的卵母细胞出现通常与成熟相关的合成模式变化。对卵泡类固醇生成的检查表明，与FSH处理的动物相比，PMSG处理的动物的卵泡中总类固醇产量，尤其是雌激素分泌高出两倍多（P<0.01）。我们得出结论，PMSG处理的动物的许多卵泡中区室发育模式和类固醇生成受到严重干扰。生殖细胞区室的过早激活会导致卵母细胞老化或异常以及生殖道环境不利。
