Edgar D H, Whalley K M, Mills J A
Department of Obstetrics and Gynaecology, University of Dundee Medical School, Nine-wells Hospital, United Kingdom.
J In Vitro Fert Embryo Transf. 1987 Oct;4(5):273-6. doi: 10.1007/BF01555203.
The effects of increasing the level of ovarian stimulation on preimplantation embryonic development were assessed using a mouse in vitro fertilization system. When F1 hybrid (C57BL/6 X CBA/Ca) mice received a single injection of 5 IU pregnant mare's serum gonadotropin (PMSG) followed 60 hr later by 5 IU human chorionic gonadotropin (hCG) approximately 50% of the resultant postovulatory oocytes developed to the blastocyst stage following in vitro fertilization. Increasing the single dose of PMSG to 10 or 15 IU resulted in significant reductions in the frequency of development to the blastocyst stage. When one or two additional doses of 5 IU PMSG were administered 24 and 48 hr after an initial injection of 5 IU, lower frequencies of oocytes with the potential for full preimplantation development were again observed. This reduction in gamete quality was significantly greater when the final dose of PMSG was administered only 12 hr prior to hCG. The results suggest that excessive gonadotropin stimulation may compromise the quality of the preimplantation embryos obtained following in vitro fertilization and that the timing of gonadotropin administration may also be critical.
利用小鼠体外受精系统评估了增加卵巢刺激水平对植入前胚胎发育的影响。当F1杂种（C57BL/6×CBA/Ca）小鼠单次注射5国际单位孕马血清促性腺激素（PMSG），60小时后再注射5国际单位人绒毛膜促性腺激素（hCG）时，约50%的排卵后卵母细胞在体外受精后发育到囊胚阶段。将PMSG的单次剂量增加到10或15国际单位会导致发育到囊胚阶段的频率显著降低。当在首次注射5国际单位PMSG后24小时和48小时再额外注射一或两剂5国际单位PMSG时，再次观察到具有完全植入前发育潜力的卵母细胞频率较低。当PMSG的最终剂量在hCG前仅12小时给药时，配子质量的这种降低明显更大。结果表明，过量的促性腺激素刺激可能会损害体外受精后获得的植入前胚胎的质量，并且促性腺激素给药的时间也可能至关重要。
