Department of Biotechnology, St. Mary's College (Autonomous), Thrissur, Kerala, 680020, India; Manipur International University, Imphal, Manipur, 795140, India.
Department of Microbiology, DKM College for Women, Vellore, Tamil Nadu, 632001, India.
Microb Pathog. 2024 Nov;196:106933. doi: 10.1016/j.micpath.2024.106933. Epub 2024 Sep 11.
Staphylococcus aureus is the most common cause of serious health conditions because of the formation of biofilm, which lowers antibiotic efficacy and enhances infection transmission and tenacious behavior. This bacteria is a major threat to the worldwide healthcare system. Silver nanoparticles have strong antibacterial characteristics and emerged as a possible alternative. This work is most relevant since it investigates the parameters influencing the biogenic nanoparticle-assisted control of bacterial biofilms by Staphylococcus aureus. Nanoparticles were fabricated utilizing Hellenia speciosa rhizome extracts, which largely comprised physiologically active components such as spirost-5-en-3-yl acetate, thymol, stigmasterol, and diosgenin, enhanced with the creation of silver nanocomposites. GC-MS, XRD, DLS, SEM, EDX, FTIR and TEM were used to investigate the characteristics of nanoparticles. The microtiter plate experiment showed that nanoparticles destroyed biofilms by up to 92.41 % at doses that ranged from 0 to 25 μg/ml. Fluorescence microscopy and SEM demonstrated the nanoparticles' capacity to prevent bacterial surface adhesion. EDX research revealed that the organic extract efficiently formed silver nanoparticles with considerable oxygen incorporation, which was attributed to phytochemicals that stabilize AgNPs and prevent accumulation. FTIR spectroscopy indicated the existence of hydroxyl, carbonyl, and carboxylate groups, which are essential for nanoparticle stability. TEM revealed that the AgNPs were spheroidal, with diameters ranging from 40 to 60 nm and an average of 46 nm. These results demonstrate the efficacy of H. speciosa extract in creating stable, well-defined AgNPs suited for a variety of applications. This work underlines the potential of green-synthesized AgNPs in biomedical applications, notably in the treatment of S. aureus biofilm-associated illnesses. The thorough characterization gives important information on the stability and efficiency of these biogenic nanoparticles.
金黄色葡萄球菌是导致严重健康问题的最常见原因，这是因为其生物膜的形成降低了抗生素的疗效，并增强了感染的传播和顽强性。这种细菌是全球医疗保健系统的主要威胁。纳米银颗粒具有很强的抗菌特性，因此成为一种可能的替代品。由于这项工作研究了影响金黄色葡萄球菌生物膜的生物成因纳米颗粒辅助控制的参数，因此具有重要意义。纳米颗粒是利用 Hellenia speciosa 根茎提取物制造的，其中包含大量生理活性成分，如螺甾-5-烯-3-基乙酸酯、百里酚、豆甾醇和薯蓣皂苷元，并通过创建银纳米复合材料进行了增强。GC-MS、XRD、DLS、SEM、EDX、FTIR 和 TEM 用于研究纳米颗粒的特性。微量滴定板实验表明，纳米颗粒在 0 至 25μg/ml 的剂量范围内可破坏高达 92.41%的生物膜。荧光显微镜和 SEM 表明纳米颗粒具有阻止细菌表面附着的能力。EDX 研究表明，有机提取物有效地形成了具有大量氧掺入的银纳米颗粒，这归因于稳定 AgNPs 并防止其聚集的植物化学物质。FTIR 光谱表明存在羟基、羰基和羧基，这对于纳米颗粒的稳定性至关重要。TEM 显示 AgNPs 为球形，直径在 40 至 60nm 之间，平均直径为 46nm。这些结果表明 H. speciosa 提取物在创建适合各种应用的稳定、定义明确的 AgNPs 方面的功效。这项工作强调了绿色合成 AgNPs 在生物医学应用中的潜力，特别是在治疗金黄色葡萄球菌生物膜相关疾病方面。全面的表征提供了有关这些生物成因纳米颗粒稳定性和效率的重要信息。
