Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran.
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Basic Microbiol. 2019 Jul;59(7):701-712. doi: 10.1002/jobm.201900096. Epub 2019 May 28.
Silver nanoparticles (AgNPs) are at the forefront of the swiftly developing scope of nanotechnology. In the current study, we investigated the green synthesis of AgNPs using Artemisia scoporia as a reducing and capping agent. The biosynthesized AgNPs were characterized using ultraviolet-visible spectroscopy, X-ray diffraction, Fourier-Transform infrared spectroscopy, dispersive absorption spectroscopy, scanning electron microscopy, and transmission electron microscopy. The efficacy of the nanoparticle synthesis was assessed by comparing the antibiofilm activity with commercial AgNPs. The effect of sub-minimum inhibitory concentrations (MICs) of AgNPs on biofilm formation was determined by microtiter plate assay. The expression level of the icaA and icaR genes was assessed by real-time polymerase chain reaction assay. The structural and functional aspects of AgNPs were confirmed. The expression levels of icaA and icaR in the isolates exposed to sub-MIC of both commercial and biosynthetic AgNPs were lower and higher than in the control group, respectively. Our results also indicated that greater reduction and induction in icaA and icaR gene expression were noticed with the sub-MIC doses of biosynthetic AgNP versus commercial AgNP, respectively. This study suggested the application of AgNPs as a significant therapeutic and clinical option in the future and usage for fabricating medical implants. Nevertheless, further investigation is required for examining the pharmaceutical and medicinal properties of AgNPs.
银纳米粒子（AgNPs）处于快速发展的纳米技术领域的前沿。在本研究中，我们使用青蒿作为还原剂和封端剂来研究 AgNPs 的绿色合成。使用紫外可见光谱、X 射线衍射、傅里叶变换红外光谱、分散吸收光谱、扫描电子显微镜和透射电子显微镜对生物合成的 AgNPs 进行了表征。通过比较抗生物膜活性与商业 AgNPs，评估了纳米颗粒合成的功效。通过微量滴定板测定法确定亚最小抑菌浓度（MICs）的 AgNPs 对生物膜形成的影响。通过实时聚合酶链反应测定法评估 icaA 和 icaR 基因的表达水平。证实了 AgNPs 的结构和功能方面。与对照组相比，暴露于商业和生物合成 AgNPs 的亚 MIC 浓度的分离株中 icaA 和 icaR 基因的表达水平较低和较高。我们的结果还表明，与商业 AgNP 相比，生物合成 AgNP 的亚 MIC 剂量下，icaA 和 icaR 基因的表达水平降低和诱导作用更大。本研究表明，AgNPs 将成为未来治疗和临床应用的重要选择，并可用于制造医疗植入物。然而，需要进一步研究以检查 AgNPs 的药物和药用特性。
