Li Xiao-Li, Jin Ye, Gao Rui, Zhou Qi-Xiu, Huang Feng, Liu Lu
Yunnan Yunzhong Institute of Nutrition and Health, College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People's Republic of China.
Yunnan Yunzhong Institute of Nutrition and Health, College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People's Republic of China.
J Ethnopharmacol. 2025 Jan 30;337(Pt 1):118818. doi: 10.1016/j.jep.2024.118818. Epub 2024 Sep 11.
Traditional Chinese Medicine (TCM) formula Wenjing Decoction (WJD) longstanding efficacy in enhancing blood circulation, resolving blood stasis, and mitigating dysmenorrhea symptoms. Despite its prevalent application, the specific mechanism underlying effect of WJD remains elusive.
The purpose of this study is to examine the material basis of Wenjing Decoction and explore the effect of WJD on rat models of dysmenorrhea with blood stasis syndrome and elucidate its mechanism.
In this study, we initially identified the chemical constituents of WJD using liquid chromatography-mass spectrometry (LC-MS). Subsequently, we employed network pharmacology to predict the mechanism of WJD in treating acute blood stasis dysmenorrhea. To further investigate the role of WJD, we established a rat model of acute blood stasis. We monitored changes in blood coagulation indexes, IL-6, TNF-α, NO, and COX-2 in rats before and after administration to confirm the successful establishment of the rat model and evaluate the therapeutic effect of WJD on dysmenorrhea and acute blood stasis. Finally, real-time fluorescence quantitative PCR (qPCR) and Western blot (WB) were utilized to investigate its mechanism.
Through LC-MS analysis, 69 chemical substances were identified in WJD. Network pharmacology study revealed that the mechanism of WJD in treating BSS may be associated with the PI3K/AKT/NF-κB pathway. Following administration, the WJD group showed gradual recovery of physical signs and coagulation index to a healthy level. Additionally, the levels of IL-6, TNF-α, and COX-2 decreased in a dose-dependent manner, whereas NO levels increased. Results from QPCR and WB detection indicated increased expression levels of p-PI3K, p-AKT, Bcl-2, and eNOS, and decreased expression levels of Bax, NFκBp65, ICAM1, and VCAM1.
The results show that WJD significantly improves the characterization, dysmenorrhea index, and coagulation-related factors in BSS rats. Through network pharmacological prediction, real-time fluorescence quantitative PCR, and Western blot analysis, it is postulated that the beneficial effects of WJD on dysmenorrhea may be linked to the PI3K/AKT/NF-κB signaling pathway. These findings offer a theoretical foundation for the advancement and utilization of WJD.
中药方剂温经汤（WJD）在促进血液循环、活血化瘀和缓解痛经症状方面具有长期疗效。尽管其应用广泛，但温经汤作用的具体机制仍不清楚。
本研究旨在研究温经汤的物质基础，探讨温经汤对血瘀证痛经大鼠模型的影响并阐明其作用机制。
在本研究中，我们首先使用液相色谱-质谱联用（LC-MS）鉴定了温经汤的化学成分。随后，我们采用网络药理学预测温经汤治疗急性血瘀型痛经的机制。为了进一步研究温经汤的作用，我们建立了急性血瘀大鼠模型。我们监测给药前后大鼠的凝血指标、IL-6、TNF-α、NO和COX-2的变化，以确认大鼠模型的成功建立，并评估温经汤对痛经和急性血瘀的治疗效果。最后，利用实时荧光定量PCR（qPCR）和蛋白质免疫印迹法（WB）研究其作用机制。
通过LC-MS分析，在温经汤中鉴定出69种化学物质。网络药理学研究表明，温经汤治疗血瘀证的机制可能与PI3K/AKT/NF-κB信号通路有关。给药后，温经汤组的体征和凝血指标逐渐恢复至健康水平。此外，IL-6、TNF-α和COX-2水平呈剂量依赖性降低，而NO水平升高。QPCR和WB检测结果表明，p-PI3K、p-AKT、Bcl-2和eNOS的表达水平升高，而Bax、NFκBp65、ICAM1和VCAM1的表达水平降低。
结果表明，温经汤显著改善了血瘀证大鼠的表征、痛经指数和凝血相关因子。通过网络药理学预测、实时荧光定量PCR和蛋白质免疫印迹分析，推测温经汤对痛经的有益作用可能与PI3K/AKT/NF-κB信号通路有关。这些发现为温经汤的进一步开发和应用提供了理论基础。
