Synergistic Effect of Two Peptaibols from Biocontrol Fungus Strain 40418 on CO-Induced Plant Resistance.

is a filamentous fungus used as a biological control agent against different plant diseases. The multifunctional secondary metabolites synthesized by , called peptaibols, have emerged as key elicitors in plant innate immunity. This study obtained a high-quality genome sequence for the strain 40418 and identified two peptaibol biosynthetic gene clusters using knockout techniques. The two gene cluster products were confirmed as trilongin AIV a (11-residue) and trilongin BI (20-residue) using liquid chromatography coupled with tandem mass spectrometry. Further investigations revealed that these peptaibols induce plant resistance to pv () DC3000 infection while triggering plant immunity and cell death. Notably, the two peptaibols exhibit synergistic effects in plant-microbe signaling interactions, with trilongin BI having a predominant role. Moreover, the induction of tomato resistance against showed similarly promising results.