Wofsy L
Scan Electron Microsc. 1979(3):565-72.
Various forms and applications of the hapten-sandwich method for labeling cell surface antigens are reviewed. This is a two-layer antibody technique: the first antibody that binds antigen has been chemically modified so that a number of hapten groups are attached; the second antibody is directed against the hapten and has been coupled to a marker for amplified detection. This methodology is particularly suited to circumstances where high sensitivity and specificity are required for labeling alloantigens or for discriminating between antigens in double-labeling procedures. It is applicable to fluorescence visualization, electron microscopy, and radioisotopic labeling. It lends itself also to procedures for specific separation of differing lymphoid cell populations, and has been adapted to studies of cell activation in the immune response.
本文综述了用于标记细胞表面抗原的半抗原夹心方法的各种形式及应用。这是一种双层抗体技术：与抗原结合的第一抗体经过化学修饰，使其附着多个半抗原基团；第二抗体针对半抗原，并与用于放大检测的标记物偶联。该方法特别适用于标记同种异体抗原或在双重标记程序中区分抗原时需要高灵敏度和特异性的情况。它适用于荧光可视化、电子显微镜和放射性同位素标记。它也适用于不同淋巴细胞群体的特异性分离程序，并已应用于免疫应答中细胞活化的研究。
