半抗原夹心标记。II. 适用于扫描电子显微镜的细胞表面标志物的免疫特异性附着。
Hapten-sandwich labeling. II. Immunospecific attachment of cell surface markers suitable for scanning electron microscopy.
作者信息
Nemanic M K, Carter D P, Pitelka D R, Wofsy L
出版信息
J Cell Biol. 1975 Feb;64(2):311-21. doi: 10.1083/jcb.64.2.311.
Abstract
A hapten-sandwich procedure has been used for immunospecific labeling of cell surface antigens with markers visible by scanning electron microscopy. Antihapten antibody was used to link hapten-modified tobacco mosaic virus, bushy stunt virus, or hemocyanin to hapten-modified human erythrocytes. The antihapten antibody bridge was also used to link the hapten-virus marker to hapten-modified antibodies against mammary tumor virus on mouse mammary tumor cells, or against immunoglobulin receptors on mouse splenic lymphocytes. In all cases, labeling was highly specific. With this technique, it is possible to (a) compare morphological features of cells bearing differing cell surface antigens, and (b) examine the arrangement of specific antigenic sites on a cell surface or their distribution relative to membrane structures such as microvilli.
摘要
一种半抗原夹心程序已被用于通过扫描电子显微镜可见的标记物对细胞表面抗原进行免疫特异性标记。抗半抗原抗体被用于将半抗原修饰的烟草花叶病毒、丛矮病毒或血蓝蛋白与半抗原修饰的人红细胞相连。抗半抗原抗体桥也被用于将半抗原-病毒标记物与半抗原修饰的针对小鼠乳腺肿瘤细胞上的乳腺肿瘤病毒或小鼠脾淋巴细胞上的免疫球蛋白受体的抗体相连。在所有情况下，标记都是高度特异性的。利用这项技术，可以（a）比较带有不同细胞表面抗原的细胞的形态特征，以及（b）检查细胞表面上特定抗原位点的排列或它们相对于诸如微绒毛等膜结构的分布。
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