Measurements of chemical carcinogen-induced sister-chromatid exchanges in a whole organ in vitro.

Sister-chromatid exchange (SCE) was measured in epithelial cells of mammary organs exposed to 3 different carcinogens in vitro. 7,12-Dimethylbenz[alpha]anthracene (DMBA), N-nitrosodiethylamine (DENA) and N-methyl-N-nitrosourea (MNU) are capable of inducing a significant enhancement of SCE over the basal level in the mammary cells. Analysis of SCE caused by the mutagenic and/or carcinogenic agents in the organ culture presents the advantages that: (1) the culture procedure does not require enzymatic dissociation of the tissue and (2) the medium does not need to be supplemented with serum or exogenous mitogenic agents thereby minimizing the basal level of SCE. Moreover, the assay does not require utilization of exogenous microsomal enzymes needed for activation of certain carcinogenic chemicals. SCE measurements in the organ culture system also should provide clues about the mechanism of action of the chemopreventive agents, particularly the antioxidants, which are under investigation for their possible influence on the processes of neoplastic transformation.