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短期精料饲喂差异对经超数排卵处理的韩牛供体母牛体内胚胎回收率的影响

Effects of Short-Term Differences in Concentrate Feeding on the Recovery of In Vivo Embryos in Hanwoo Donor Cows through Superovulation Treatment.

作者信息

Ha Seungmin, Kim Namtae, Park Mi-Ryung, Lee Seyoung, Cho Sang-Rae, Song Huimang, Jin Daehyeok, Kim Ui-Hyung, Ko Yeoung-Gyu

机构信息

Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Republic of Korea.

Hanwoo Research Institute, National Institute of Animal Science, Rural Development Administration, Pyeongchang 25340, Republic of Korea.

出版信息

Animals (Basel). 2024 Sep 6;14(17):2591. doi: 10.3390/ani14172591.

Abstract

Superovulation is a technique used to increase the number of oocytes released for fertilization. This study investigated the effects of short-term differences in concentrate feed intake on in vivo embryo production through superovulation in indigenous Korean (Hanwoo) cows. The cows were given fresh water and hay ad libitum and randomly divided into three groups (control (CON, n = 9): 2.0 kg/day (unchanged diet); low concentrate (LC, n = 10): 0 kg/day; and high concentrate (HC, n = 8): 4.0 kg/day) according to the amount of formula they were fed. This feeding treatment began seven days before the start of the hormonal treatment for superovulation. From the results, the LC group had the greatest weight change and the lowest body condition score at harvest, followed by the CON and HC groups ( < 0.05). The LC group had the highest number of harvesting embryos, followed by the HC and CON groups ( < 0.05). Estradiol, progesterone, glucose, total cholesterol, high-density lipoprotein, low-density lipoprotein, total protein, and blood urea nitrogen concentrations did not differ between the groups, except for a temporary increase in the HC group on day 0. These findings suggest that more embryos may be harvested when short-term changes in concentrate intake are made during superovulatory responses in Hanwoo cows.

摘要

超数排卵是一种用于增加释放用于受精的卵母细胞数量的技术。本研究调查了韩国本土(韩牛)母牛超数排卵期间精饲料采食量的短期差异对体内胚胎生产的影响。给母牛随意提供淡水和干草,并根据所喂配方饲料的量将其随机分为三组(对照组(CON,n = 9):2.0千克/天(饮食不变);低精料组(LC,n = 10):0千克/天;高精料组(HC,n = 8):4.0千克/天)。这种饲养处理在超数排卵激素处理开始前7天开始。结果显示,LC组在收获时体重变化最大,体况评分最低,其次是CON组和HC组(P<0.05)。LC组收获的胚胎数量最多,其次是HC组和CON组(P<0.05)。除了第0天HC组暂时升高外,各组之间雌二醇、孕酮、葡萄糖、总胆固醇、高密度脂蛋白、低密度脂蛋白、总蛋白和血尿素氮浓度没有差异。这些发现表明,在韩牛母牛超数排卵反应期间进行精料摄入量的短期改变时,可能收获更多的胚胎。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9148/11393984/6767e7cdaca4/animals-14-02591-g001.jpg

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