Republic of Turkish Ministry of Agriculture and Forestry General Directorate of Livestock, Ankara, Turkey.
International Center for Livestock Research and Training, Ankara, Turkey.
Reprod Domest Anim. 2024 Jun;59(6):e14629. doi: 10.1111/rda.14629.
This study aims to investigate the relationship between metabolic parameters and the number of embryos produced in superovulated cows with high genetic characteristics in milk yield. Eighteen Holstein donors were treated with classic superovulation protocols, AI and flushing. During superovulation, decreasing doses of FSH (follicle-stimulating hormone) were administered at 12-h intervals for 4 days. Plasma insulin-like growth factor (IGF1), glucose (GLU), beta-hydroxybutyric acid (BHB), non-esterified fatty acid (NEFA), blood urea nitrogen (BUN) and total protein (TP) levels were determined by using an autoanalyzer. The mixed model analysis of variance was used for statistical analysis. As a result, plasma IGF1, BHB and BUN had significant interactions with both groups and days (p < .05). Additionally, plasma TP-days interactions were significant (p < .05). Furthermore, there was a negative correlation between the number of embryos and plasma BHB levels (p < .05). In conclusion, under appropriate environmental conditions, metabolic profile control of donors can contribute to the embryo production process and to the studies on the metabolic infrastructure.
本研究旨在探讨具有高产奶遗传特性的超排奶牛的代谢参数与胚胎数量之间的关系。18 头荷斯坦供体采用经典超排方案、人工授精和冲胚进行处理。在超排期间,每隔 12 小时给予递减剂量的促卵泡素(FSH),持续 4 天。使用自动分析仪测定血浆胰岛素样生长因子(IGF1)、葡萄糖(GLU)、β-羟丁酸(BHB)、非酯化脂肪酸(NEFA)、血尿素氮(BUN)和总蛋白(TP)水平。采用混合模型方差分析进行统计分析。结果表明,血浆 IGF1、BHB 和 BUN 与两组和天数均有显著的互作(p<0.05)。此外,血浆 TP-天数的互作也有显著差异(p<0.05)。此外,胚胎数量与血浆 BHB 水平呈负相关(p<0.05)。总之,在适当的环境条件下,供体的代谢谱控制有助于胚胎的产生过程,并有助于研究代谢基础结构。