Department of Anesthesiology, Medical Faculty, University Hospital Heidelberg, University Heidelberg, Im Neuenheimer Feld 420, 69120 Heidelberg, Germany.
Hospital Bergstrasse, 64646 Heppenheim, Germany.
Int J Mol Sci. 2024 Aug 27;25(17):9283. doi: 10.3390/ijms25179283.
The clinical effectiveness of Oxiris, particularly in reducing cytokines, remains uncertain due to the limited data provided. This study explored and analyzed the application value of Oxiris endotoxin adsorption technology in a large animal model. Pigs received an intravenous LPS infusion. Six animals were treated 2 h after the infusion with an Oxiris hemadsorption using a pumpless extracorporeal technique for 6 h. Five animals served as controls. Cardiocirculatory parameters, hyperspectral analysis, and a panel of cytokines were measured. The lipopolysaccharide infusion induced sepsis-like inflammation with tachycardia, elevated pulmonary pressure, elevated lactate level, as well as elevated pro-inflammatory cytokines like interferon (IFN)-γ, interleukin (IL)-1β, IL-2, IL-6, IL-8, IL-12 and tumor necrosis factor alpha (TNF-α). In addition, increases of anti-inflammatory cytokines like IL-1ra and IL-10 were found. After 3 and 6 h in both groups, pro-inflammatory cytokines were significantly reduced. No differences between the intervention and the control group could be detected after 3 and 6 h for IL-1β, IL-2, IL-6, IL-8, IL-12 and TNF-α, suggesting no effect of the Oxiris filter on the elimination of elevated cytokines with a pumpless extracorporeal hemadsorption technique. The presented large animal model may be a promising option for studying the effects of hemadsorption techniques.
由于提供的数据有限，奥昔里斯在减少细胞因子方面的临床疗效尚不确定。本研究探讨和分析了奥昔里斯内毒素吸附技术在大型动物模型中的应用价值。猪接受静脉内 LPS 输注。在输注后 2 小时，6 只动物使用无泵体外技术进行 6 小时的奥昔里斯血液吸附治疗。5 只动物作为对照。测量心循环参数、高光谱分析和细胞因子谱。脂多糖输注引起类败血症炎症，表现为心动过速、肺动脉压升高、乳酸水平升高以及促炎细胞因子如干扰素（IFN）-γ、白细胞介素（IL）-1β、IL-2、IL-6、IL-8、IL-12 和肿瘤坏死因子-α（TNF-α）升高。此外，还发现抗炎细胞因子如 IL-1ra 和 IL-10 增加。在两组中，在 3 和 6 小时后，促炎细胞因子均显著降低。在 3 和 6 小时后，干预组和对照组之间的 IL-1β、IL-2、IL-6、IL-8、IL-12 和 TNF-α无差异，表明无泵体外血液吸附技术对清除升高的细胞因子无奥昔里斯过滤器的作用。所提出的大型动物模型可能是研究血液吸附技术效果的有前途的选择。
