College of Chemistry, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China.
Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China.
Int J Mol Sci. 2024 Aug 28;25(17):9340. doi: 10.3390/ijms25179340.
Platelet-rich plasma (PRP) has become an important regenerative therapy. However, the preparation method of PRP has not been standardized, and the optimal platelet concentration for PRP used in skin wound repair is unclear, leading to inconsistent clinical efficacy of PRP. Therefore, the development of standardized preparation methods for PRP and the investigation of the dose-response relationship between PRP with different platelet concentrations and tissue regeneration plays an important role in the development and clinical application of PRP technology. This study has developed an integrated blood collection device from blood drawing to centrifugation. Response surface methodology was employed to optimize the preparation conditions, ultimately achieving a platelet recovery rate as high as 95.74% for PRP (with optimal parameters: centrifugation force 1730× , centrifugation time 10 min, and serum separation gel dosage 1.4 g). Both in vitro and in vivo experimental results indicate that PRP with a (2×) enrichment ratio is the most effective in promoting fibroblast proliferation and skin wound healing, with a cell proliferation rate of over 150% and a wound healing rate of 78% on day 7.
富血小板血浆（PRP）已成为一种重要的再生治疗方法。然而，PRP 的制备方法尚未标准化，用于皮肤创伤修复的 PRP 的最佳血小板浓度也不清楚，导致 PRP 的临床疗效不一致。因此，开发标准化的 PRP 制备方法以及研究不同血小板浓度的 PRP 与组织再生之间的剂量反应关系，对于 PRP 技术的发展和临床应用具有重要意义。本研究从采血到离心开发了一种集成血液采集装置。采用响应面法优化了制备条件，最终使 PRP 的血小板回收率高达 95.74%（最佳参数：离心力 1730×g，离心时间 10 分钟，血清分离凝胶剂量 1.4 g）。体外和体内实验结果均表明，（2×）浓缩比的 PRP 促进成纤维细胞增殖和皮肤伤口愈合的效果最佳，细胞增殖率超过 150%，第 7 天伤口愈合率达到 78%。
