Laboratory of Aging Research, School of Medicine, University of Electronic Science and Technology of China, Chengdu 611731, China.
Institute for Toxicology, Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Int J Mol Sci. 2024 Sep 6;25(17):9675. doi: 10.3390/ijms25179675.
Glycation Stress (GS), induced by advanced glycation end-products (AGEs), significantly impacts aging processes. This study introduces a new model of GS of by feeding them OP50 cultured in a glucose-enriched medium, which better simulates human dietary glycation compared to previous single protein-glucose cross-linking methods. Utilizing WormCNN, a deep learning model, we assessed the health status and calculated the Healthy Aging Index (HAI) of worms with or without GS. Our results demonstrated accelerated aging in the GS group, evidenced by increased autofluorescence and altered gene expression of key aging regulators, and . Additionally, we observed elevated pharyngeal pumping rates in AGEs-fed worms, suggesting an addictive response similar to human dietary patterns. This study highlights the profound effects of GS on worm aging and underscores the critical role of computer vision in accurately assessing health status and aiding in the establishment of disease models. The findings provide insights into glycation-induced aging and offer a comprehensive approach to studying the effects of dietary glycation on aging processes.
糖基化应激（GS）是由晚期糖基化终产物（AGEs）引起的，它对衰老过程有显著影响。本研究通过用富含葡萄糖的培养基培养 OP50 来喂养秀丽隐杆线虫，从而建立了一种新的 GS 模型，与之前的单一蛋白-葡萄糖交联方法相比，这种方法更好地模拟了人类饮食中的糖基化。我们利用深度学习模型 WormCNN 评估了有或没有 GS 的线虫的健康状况，并计算了健康衰老指数（HAI）。我们的结果表明，GS 组的线虫出现了加速衰老的现象，这表现为自发荧光增加和关键衰老调节基因 和 的表达改变。此外，我们还观察到 AGEs 喂养的线虫的咽部抽吸率升高，这表明存在类似于人类饮食模式的成瘾反应。这项研究强调了 GS 对线虫衰老的深远影响，并突出了计算机视觉在准确评估健康状况和辅助建立疾病模型方面的关键作用。研究结果为糖基化诱导的衰老提供了新的见解，并提供了一种全面的方法来研究饮食糖基化对衰老过程的影响。
