Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Agricultural College, Guangxi University, Nanning 530004, China.
Int J Mol Sci. 2024 Sep 9;25(17):9743. doi: 10.3390/ijms25179743.
Cytoplasmic male sterility (CMS) is important for commercial hybrid seed production. However, it is still not used in eggplant ( L.), and corresponding regulatory genes and mechanisms of action have not been reported. We report CMS line 327A, which was derived from the hybridization between cultivated and wild eggplants. By looking at different stages of anther development under a microscope, we saw that the 327A anther's tapetum layer vacuolized during meiosis, which caused abortion. To investigate the 327A CMS regulatory genes, the mitochondrial genomes of 327A and its maintainer line 327B were assembled de novo. It was found that 15 unique ORFs (Open Reading Frame) were identified in 327A. RT-PCR and RT-QPCAR tests confirmed that and , 327A-specific ORFs with a transmembrane domain, were strongly expressed in sterile anthers of 327A. In addition, has a chimeric structure with the ribosomal protein subunit . Therefore, and can be considered strong candidate genes for CMS. Concurrently, we analyzed the characteristics of CMS to develop a functional molecular marker, CMS312, targeting a future theoretical basis for eggplant CMS three-line molecular breeding.
细胞质雄性不育（CMS）对商业杂交种子生产很重要。然而，茄子（L.）中尚未应用 CMS，也没有报道相应的调控基因和作用机制。我们报告了 CMS 系 327A，它是由栽培茄子和野生茄子杂交产生的。通过在显微镜下观察不同阶段的花药发育，我们发现 327A 花药的绒毡层在减数分裂过程中出现空泡化，导致败育。为了研究 327A CMS 的调控基因，我们从头组装了 327A 及其保持系 327B 的线粒体基因组。结果发现 327A 中有 15 个独特的开放阅读框（ORF）。RT-PCR 和 RT-QPCR 测试证实，具有跨膜结构域的 327A 特异性 ORF 和 ，在 327A 的不育花药中强烈表达。此外， 与核糖体蛋白亚基 具有嵌合结构。因此，可以认为 和 是 CMS 的候选强基因。同时，我们分析了 CMS 的特征，开发了一个功能性分子标记 CMS312，为茄子 CMS 三系分子育种提供了未来的理论基础。
