Hubei Key Laboratory of Nutritional Quality and Safety of Agro-Products, Institute of Quality Standard and Testing Technology for Agro-Products, Hubei Academy of Agricultural Sciences, Wuhan 430072, China.
Ministry of Agriculture Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2024 Sep 9;25(17):9757. doi: 10.3390/ijms25179757.
Rapeseed is an important oil crop in the world. Wood vinegar could increase the yield and abiotic resistance of rapeseed. However, little is known about the underlying mechanisms of wood vinegar or its valid chemical components on rapeseed. In the present study, wood vinegar and butyrolactone (γ-Butyrolactone, one of the main components of wood vinegar) were applied to rapeseed at the seedling stage, and the molecular mechanisms of wood vinegar that affect rapeseed were studied by combining transcriptome and metabolomic analyses. The results show that applying wood vinegar and butyrolactone increases the biomass of rapeseed by increasing the leaf area and the number of pods per plant, and enhances the tolerance of rapeseed under low temperature by reducing membrane lipid oxidation and improving the content of chlorophyll, proline, soluble sugar, and antioxidant enzymes. Compared to the control, 681 and 700 differentially expressed genes were in the transcriptional group treated with wood vinegar and butyrolactone, respectively, and 76 and 90 differentially expressed metabolites were in the metabolic group. The combination of transcriptome and metabolomic analyses revealed the key gene-metabolic networks related to various pathways. Our research shows that after wood vinegar and butyrolactone treatment, the amino acid biosynthesis pathway of rapeseed may be involved in mediating the increase in rapeseed biomass, the proline metabolism pathway of wood vinegar treatment may be involved in mediating rapeseed's resistance to low-temperature stress, and the sphingolipid metabolism pathway of butyrolactone treatment may be involved in mediating rapeseed's resistance to low-temperature stress. It is suggested that the use of wood vinegar or butyrolactone are new approaches to increasing rapeseed yield and low-temperature resistance.
油菜是世界上重要的油料作物。木醋液可以提高油菜的产量和抗逆性。然而，关于木醋液或其有效化学成分对油菜的潜在机制知之甚少。在本研究中，木醋液和丁内酯（γ-丁内酯，木醋液的主要成分之一）在油菜幼苗期使用，通过结合转录组和代谢组分析研究了影响油菜的木醋液的分子机制。结果表明，通过增加叶面积和每株角果数，木醋液和丁内酯增加了油菜的生物量，通过降低膜脂质氧化和提高叶绿素、脯氨酸、可溶性糖和抗氧化酶的含量，增强了油菜在低温下的耐受性。与对照相比，木醋液和丁内酯处理的转录组分别有 681 个和 700 个差异表达基因，代谢组分别有 76 个和 90 个差异表达代谢物。转录组和代谢组分析的结合揭示了与各种途径相关的关键基因-代谢网络。我们的研究表明，木醋液和丁内酯处理后，油菜的氨基酸生物合成途径可能参与调节油菜生物量的增加，木醋液处理的脯氨酸代谢途径可能参与调节油菜对低温胁迫的抗性，而丁内酯处理的鞘脂代谢途径可能参与调节油菜对低温胁迫的抗性。建议使用木醋液或丁内酯是提高油菜产量和抗低温的新方法。
