通过交叉免疫电泳对结核分枝杆菌单克隆抗体的特异性进行表征。

Characterization of the specificity of monoclonal antibodies against Mycobacterium tuberculosis by crossed immunoelectrophoresis.

作者信息

Harboe M, Coates A R, Hewitt J

出版信息

Scand J Immunol. 1985 Jul;22(1):93-8. doi: 10.1111/j.1365-3083.1985.tb01863.x.

DOI:10.1111/j.1365-3083.1985.tb01863.x

Abstract

The specificity of monoclonal antibodies against Mycobacterium tuberculosis was determined by crossed immunoelectrophoresis. Radiolabelled, non-precipitating monoclonal antibody was incorporated in the top gel together with unlabelled polyvalent, precipitating anti-bacillus Calmette-Guérin (BCG) antibody. Inclusion of labelled antibody in individual precipitate lines was demonstrated by autoradiography. Each monoclonal antibody was localized in a single precipitate line: antibody TB73 reacted with BCG antigen 56; TB71 and TB72 reacted with BCG antigen 78; and TB78 reacted with BCG antigen 82. The technique permits precise determination of the specificity of monoclonal antibodies at the level of reactivity with native immunogenic components of complex microorganisms without prior antigen purification.

摘要

通过交叉免疫电泳确定了抗结核分枝杆菌单克隆抗体的特异性。将放射性标记的、不沉淀的单克隆抗体与未标记的多价、沉淀性抗卡介苗（BCG）抗体一起加入上层凝胶中。通过放射自显影证明了标记抗体在各个沉淀线中的存在。每种单克隆抗体都定位在一条单一的沉淀线中：抗体TB73与BCG抗原56反应；TB71和TB72与BCG抗原78反应；TB78与BCG抗原82反应。该技术无需事先进行抗原纯化，就能在与复杂微生物的天然免疫原性成分反应的水平上精确测定单克隆抗体的特异性。