Morris J A, Ivanyi J
J Med Microbiol. 1985 Jun;19(3):367-73. doi: 10.1099/00222615-19-3-367.
Antigen extracts obtained by sonication of 22 strains of Mycobacterium bovis from cattle and badgers together with extracts of strains of M. tuberculosis, M. paratuberculosis, M. avium, M. africanum, M. kansasi, M. leprae and BCG were examined with a panel of 10 monoclonal antibodies to M. tuberculosis or M. leprae. Antigen extracts were coated in aqueous solution (wet coating) and the extracts were also dried on to the polyvinyl plates (dry coating). When dry coating was compared to wet coating, there was a major increase in the binding of monoclonal antibody ML03 to M. avium and M. paratuberculosis, monoclonal antibody ML02 to M. paratuberculosis, and monoclonal antibodies TB71 and TB72 to the majority of M. bovis isolates. The study confirmed that on wet-coated plates, monoclonal antibodies TB71 and TB72 bind poorly or not at all to M. bovis and that monoclonal antibodies TB68, TB78, TB77 and TB23 each bind to field strains of M. bovis while TB23 binds poorly to BCG in wet-coating conditions. Antibodies TB72 and TB71, originally thought to be specific for M. tuberculosis, each reacted with M. africanum. Antibody TB78 bound to M. paratuberculosis but did not react with M. avium, and M. avium and M. paratuberculosis were distinguished from M. bovis and M. tuberculosis by the binding of antibody ML03 to dry-coated plates. When wet-coated plates were used, ML03 bound strongly only to M. leprae. The panel of monoclonal antibodies did not demonstrate distinct serotype differences between the field isolates of M. bovis.
通过超声处理从牛和獾身上获取的22株牛分枝杆菌菌株的抗原提取物，连同结核分枝杆菌、副结核分枝杆菌、鸟分枝杆菌、非洲分枝杆菌、堪萨斯分枝杆菌、麻风分枝杆菌和卡介苗菌株的提取物，用一组针对结核分枝杆菌或麻风分枝杆菌的10种单克隆抗体进行检测。抗原提取物以水溶液形式包被（湿包被），提取物也干燥在聚乙烯板上（干包被）。当将干包被与湿包被进行比较时，单克隆抗体ML03与鸟分枝杆菌和副结核分枝杆菌的结合、单克隆抗体ML02与副结核分枝杆菌的结合以及单克隆抗体TB71和TB72与大多数牛分枝杆菌分离株的结合都有显著增加。该研究证实，在湿包被板上，单克隆抗体TB71和TB72与牛分枝杆菌的结合很差或根本不结合，并且单克隆抗体TB68、TB78、TB77和TB23各自与牛分枝杆菌的野外菌株结合，而在湿包被条件下，TB23与卡介苗的结合很差。抗体TB72和TB71最初被认为是结核分枝杆菌特异性的，它们都与非洲分枝杆菌反应。抗体TB78与副结核分枝杆菌结合，但不与鸟分枝杆菌反应，并且通过抗体ML03与干包被板的结合，鸟分枝杆菌和副结核分枝杆菌与牛分枝杆菌和结核分枝杆菌得以区分。当使用湿包被板时，ML03仅与麻风分枝杆菌强烈结合。这组单克隆抗体未显示牛分枝杆菌野外分离株之间有明显的血清型差异。
