Laboratory of Exercise and Nutrition, Department of Sports Medicine and Science in Graduate School, Konkuk University, Seoul 05029, Republic of Korea.
Physical Activity and Performance Institute (PAPI), Konkuk University, Seoul 05029, Republic of Korea.
Nutrients. 2024 Aug 24;16(17):2831. doi: 10.3390/nu16172831.
Recent studies suggest that lactate intake has a positive effect on glycogen recovery after exercise. However, it is important to verify the effect of lactate supplementation alone and the timing of glycogen recovery. Therefore, in this study, we aimed to examine the effect of lactate supplementation immediately after exercise on glycogen recovery in mice liver and skeletal muscle at 1, 3, and 5 h after exercise. Mice were randomly divided into the sedentary, exercise-only, lactate, and saline-treated groups. mRNA expression and activation of glycogen synthesis and lactate transport-related factors in the liver and skeletal muscle were assessed using real-time polymerase chain reaction. Skeletal muscle glycogen concentration showed an increasing trend in the lactate group compared with that in the control group at 3 and 5 h after post-supplementation. Additionally, exogenous lactate supplementation significantly increased the expression of core glycogen synthesis enzymes, lactate transporters, and pyruvate dehydrogenase E1 alpha 1 in the skeletal muscles. Conversely, glycogen synthesis, lactate transport, and glycogen oxidation to acetyl-CoA were not significantly affected in the liver by exogenous lactate supplementation. Overall, these results suggest that post-exercise lactate supplement enables glycogen synthesis and recovery in skeletal muscles.
最近的研究表明，运动后摄入乳酸对糖原的恢复有积极影响。然而，单独验证乳酸补充的效果以及糖原恢复的时间非常重要。因此，在这项研究中，我们旨在研究运动后立即补充乳酸对运动后 1、3 和 5 小时小鼠肝脏和骨骼肌中糖原恢复的影响。小鼠被随机分为安静组、运动组、乳酸组和盐水处理组。使用实时聚合酶链反应评估肝脏和骨骼肌中与糖原合成和乳酸转运相关的因子的 mRNA 表达和激活。与对照组相比，补充乳酸组在补充后 3 小时和 5 小时时骨骼肌糖原浓度呈上升趋势。此外，外源性乳酸补充显著增加了骨骼肌中核心糖原合成酶、乳酸转运体和丙酮酸脱氢酶 E1α1 的表达。相反，外源性乳酸补充对肝脏中糖原的合成、乳酸的转运以及糖原氧化为乙酰辅酶 A 没有显著影响。总的来说，这些结果表明，运动后补充乳酸可促进骨骼肌中糖原的合成和恢复。
