Hwang Deunsol, Kim Taeho, Kyun Sunghwan, Jang Inkwon, Park Hun-Young, Kim Sung-Woo, Han Jin-Soo, So Jae-Moo, Lee Chi-Ho, Park Jonghoon, Lim Kiwon, Kim Jisu
Physical Activity and Performance Institute, Konkuk University, Seoul, Republic of Korea.
Department of Sports Medicine and Science in Graduate School, Konkuk University, Seoul, Republic of Korea.
Phys Act Nutr. 2025 Jun;29(2):26-34. doi: 10.20463/pan.2025.0011. Epub 2025 Jun 30.
Oxamate is a well-known inhibitor of glycolysis. However, its broad inhibitory effects on whole-body energy metabolism in vivo have not been identified. Therefore, we aimed to investigate its effects on wholebody energy metabolism in mice.
Ten-week-old male ICR mice were used in this study. The resting metabolic rate was measured for 3 h immediately after the intraperitoneal injection of oxamate (750 mg/kg) using a metabolic chamber system. In addition, resting blood glucose and lactate concentrations were measured. Next, the metabolism during exercise (10-25 m/min) was measured for 30 min immediately after oxamate injection using a metabolic treadmill chamber system. Post-exercise blood lactate concentrations were measured immediately after exercise sessions.
The resting respiratory exchange rate remained unchanged, but fat and carbohydrate oxidation and energy expenditure (p = 0.003, 0.049, and 0.002, respectively) were significantly suppressed following oxamate injection. While the resting blood glucose levels were significantly reduced (p = 0.002), the lactate levels were significantly elevated (p = 0.005). The respiratory exchange rate during exercise significantly increased by oxamate injection (p = 0.02). Although fat oxidation during exercise significantly reduced (p = 0.009), carbohydrate oxidation remained unchanged. Consequently, energy expenditure during exercise was significantly reduced (p = 0.024) and post-exercise blood lactate levels were significantly elevated (p = 0.005) by oxamate injection.
Oxamate suppressed whole-body energy metabolism by inhibiting fat oxidation and altering lactate dynamics in vivo. These results provide novel insights into the systemic metabolic effects of oxamate and highlight the need for further investigation of its impact under different physiological conditions.
草氨酸盐是一种著名的糖酵解抑制剂。然而，其对体内全身能量代谢的广泛抑制作用尚未明确。因此，我们旨在研究其对小鼠全身能量代谢的影响。
本研究使用10周龄雄性ICR小鼠。使用代谢室系统，在腹腔注射草氨酸盐（750mg/kg）后立即测量3小时的静息代谢率。此外，测量静息血糖和乳酸浓度。接下来，使用代谢跑步机室系统，在注射草氨酸盐后立即测量运动期间（10 - 25米/分钟）30分钟的代谢情况。运动后立即测量运动后血乳酸浓度。
静息呼吸交换率保持不变，但注射草氨酸盐后脂肪和碳水化合物氧化以及能量消耗（分别为p = 0.003、0.049和0.002）显著受到抑制。静息血糖水平显著降低（p = 0.002），而乳酸水平显著升高（p = 0.005）。注射草氨酸盐后运动期间的呼吸交换率显著增加（p = 0.02）。虽然运动期间脂肪氧化显著减少（p = 0.009），但碳水化合物氧化保持不变。因此，注射草氨酸盐后运动期间的能量消耗显著降低（p = 0.024），运动后血乳酸水平显著升高（p = 0.005）。
草氨酸盐通过抑制脂肪氧化和改变体内乳酸动态来抑制全身能量代谢。这些结果为草氨酸盐的全身代谢作用提供了新的见解，并强调了在不同生理条件下进一步研究其影响的必要性。
