通过组织化学方法对β-半乳糖苷酶缺乏症进行互补分析。

Complementation analysis of beta-galactosidase deficiency by means of histochemical method.

作者信息

Minami R, Wagatsuma K, Ishikawa Y, Watanabe M, Kusano T, Nakao T, Tsugawa S

出版信息

Tohoku J Exp Med. 1985 Jun;146(2):181-7. doi: 10.1620/tjem.146.181.

DOI:10.1620/tjem.146.181

Abstract

Cultured skin fibroblasts from 4 beta-galactosidase-deficient patients with different clinical features were hybridized and beta-galactosidase activities were measured using 4-methylumbelliferyl (4MU)-derivate and by means of indigogenic method. Compared with the assays of beta-galactosidase activity using 4MU-derivate, more clear-cut evidence was obtained in the indigogenic method for a judgement of complementation.

摘要

对4名具有不同临床特征的β-半乳糖苷酶缺陷患者的培养皮肤成纤维细胞进行杂交，并使用4-甲基伞形酮（4MU）衍生物并通过产色法测量β-半乳糖苷酶活性。与使用4MU衍生物测定β-半乳糖苷酶活性相比，产色法在判断互补性方面获得了更明确的证据。