Rozanov A Ia, Karpov L M, Petrov S A
Ukr Biokhim Zh (1978). 1985 May-Jun;57(3):71-4.
Coenzymes introduced in the ratio, peculiar for pyruvate dehydrogenase complex into the medium containing fresh-isolated mitochondria and oxidation substrate--pyruvate increase accumulation of [35S] lipoate by these organelles. This process is highly stimulated by introducing either the only CoA or a coenzyme mixture (CoA, thiamine pyrophosphate, FAD, NAD). Addition of phosphate-extracted components of mitochondria and their protein fraction with coenzymes in the ratio indicated above provides maximum accumulation of [35S] lipoate by liver mitochondria. An equimolar mixture of coenzymes as well as protein components evoke no reliable variations in [35S] lipoate accumulation by albino rat liver mitochondria, while addition of the only thiamine pyrophosphate decreases this accumulation. Reconstruction of multienzyme complexes of coenzymes and apoenzymes on mitochondrion membranes accounts for the results obtained.
以丙酮酸脱氢酶复合体特有的比例将辅酶引入含有新鲜分离线粒体和氧化底物——丙酮酸的培养基中，会增加这些细胞器对[35S]硫辛酸的积累。通过引入单独的辅酶A或辅酶混合物（辅酶A、硫胺素焦磷酸、黄素腺嘌呤二核苷酸、烟酰胺腺嘌呤二核苷酸），这个过程会受到极大刺激。以上述比例添加线粒体的磷酸盐提取物成分及其蛋白质部分与辅酶，可使肝线粒体对[35S]硫辛酸的积累达到最大值。辅酶以及蛋白质成分的等摩尔混合物不会引起白化大鼠肝线粒体对[35S]硫辛酸积累的可靠变化，而单独添加硫胺素焦磷酸会减少这种积累。辅酶和脱辅酶在线粒体膜上重建多酶复合体解释了所获得的结果。
