Shkap V, Ungar-Waron H, Pipano E, Greenblatt C
Vet Immunol Immunopathol. 1985 May;9(1):53-7. doi: 10.1016/0165-2427(85)90129-1.
Specific precipitable antibodies of both IgG and IgM classes were detected in sera of cattle naturally infected with B. besnoiti. The amount of specific antibodies of the IgG class precipitated by soluble antigen was in the range of 17-50 micrograms/ml serum while that of the IgM class ranged between 5 and 24 micrograms/ml serum. Specific antibodies precipitated by live B. besnoiti parasites were in amounts of 10 to 22 micrograms/ml serum for IgG and 4 to 26 micrograms/ml serum for IgM. Different ratios of IgG/IgM were obtained by the two methods of precipitation. This might indicate that antibodies to B. besnoiti of the IgM class can be precipitated and detected in sera of naturally infected cattle in similar amounts either by live parasites or by solubilized antigen, whereas antibodies of the IgG class can be preferentially detected when solubilized antigen is used for precipitation.
在自然感染贝氏巴贝斯虫的牛血清中检测到了IgG和IgM两类特异性可沉淀抗体。可溶性抗原沉淀的IgG类特异性抗体量在血清中为17 - 50微克/毫升，而IgM类在血清中的量在5至24微克/毫升之间。活的贝氏巴贝斯虫寄生虫沉淀的特异性抗体，IgG在血清中的量为10至22微克/毫升，IgM为4至26微克/毫升。两种沉淀方法获得了不同的IgG/IgM比率。这可能表明，IgM类针对贝氏巴贝斯虫的抗体，在自然感染牛的血清中，无论是通过活寄生虫还是通过溶解抗原，都能以相似的量被沉淀和检测到，而当使用溶解抗原进行沉淀时，IgG类抗体能被优先检测到。
