Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC, USA, 27695.
Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC, USA, 27695.
J Mech Behav Biomed Mater. 2024 Dec;160:106739. doi: 10.1016/j.jmbbm.2024.106739. Epub 2024 Sep 11.
Transcatheter arterial embolization is a minimally invasive intervention process in which the blood supply to a tumor or an abnormal area of tissue is blocked. One of the most commonly used embolic agents in clinics is microsphere (MS). In order to understand the flow behavior of microspheres in arteries, it is essential to study their mechanical properties systematically. In this work, calcium-alginate MSs with varying calcium concentrations were synthesized using a coaxial airflow method. Indocyanine green (ICG) was added as a fluorescent dye. The effect of ICG concentration change on microspheres was investigated by studying morphology, imageability, rheology, and swelling behavior. Then the effect of calcium chloride concentration change on microspheres was studied by conducting rheological tests, atomic force microscopy tests, hemolysis assay, and thrombogenicity assay. Results showed that microspheres with higher ICG concentrations have longer lasting fluorescence and lower storage modulus (G'). Higher concentrations of calcium chloride led to higher G', while the local Young's modulus obtained by AFM test was not significantly affected. The MSs with and without ICG showed good hemocompatibility and thrombogenicity.
经导管动脉栓塞术是一种微创介入过程，通过该过程阻断肿瘤或组织异常部位的血液供应。在临床上，最常用的栓塞剂之一是微球（MS）。为了了解微球在动脉中的流动行为，有必要系统地研究其力学性能。在这项工作中，使用同轴气流法合成了具有不同钙离子浓度的海藻酸钙 MS，并添加了吲哚菁绿（ICG）作为荧光染料。通过研究形态、成像性、流变学和溶胀行为，研究了 ICG 浓度变化对微球的影响。然后通过流变学测试、原子力显微镜测试、溶血试验和血栓形成试验研究了氯化钙浓度变化对微球的影响。结果表明，ICG 浓度较高的微球具有更长的荧光持续时间和更低的储能模量（G'）。较高浓度的氯化钙导致 G'更高，而通过 AFM 测试获得的局部杨氏模量没有明显影响。含有和不含有 ICG 的 MS 具有良好的血液相容性和血栓形成性。
