Henan Engineering Research Center of Industrial Recirculating Water Treatment, College of Chemistry and Molecular Sciences, Henan University, Kaifeng 475004, PR China.
School of Pharmacy, Henan University, Kaifeng, Henan 475004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jan 15;325:125105. doi: 10.1016/j.saa.2024.125105. Epub 2024 Sep 7.
As an important parameter of the cellular microenvironment, the changes in mitochondrial viscosity are closely related to various life activities. Therefore, the development of fluorescent probes for test the changes of mitochondrial viscosity has great significance. In this study, we developed two fluorescent probes for the detection of the mitochondrial viscosity changes. The probes exhibited different fluorescence intensities at different viscosity based on the twisted intramolecular charge transfer process. The characteristics of high anti-interference performance, wide pH applicability, low cytotoxicity and excellent mitochondrial targeting performance made the probes successfully used to distinguish normal cells from cancer cells, achieving visualization of viscosity changes. Furthermore, probes P1 and P2 can also be used as early diagnosis of tumors in mice and reveal the pathology of tumor development. The probes could be serve as a promising viscosity detection tool for discriminating normal cells and cancer cells in biology-related research.
作为细胞微环境的一个重要参数，线粒体粘度的变化与各种生命活动密切相关。因此，开发用于检测线粒体粘度变化的荧光探针具有重要意义。在本研究中，我们开发了两种用于检测线粒体粘度变化的荧光探针。探针基于扭曲的分子内电荷转移过程，在不同粘度下表现出不同的荧光强度。高抗干扰性能、宽 pH 适用性、低细胞毒性和优异的线粒体靶向性能的特点，使探针成功用于区分正常细胞和癌细胞，实现了粘度变化的可视化。此外，探针 P1 和 P2 还可用于小鼠肿瘤的早期诊断，并揭示肿瘤发展的病理学。这些探针可以作为一种有前途的粘度检测工具，用于区分生物学研究中的正常细胞和癌细胞。
