Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand; Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Bangkok, 10700, Thailand.
Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand.
Talanta. 2025 Jan 1;281:126841. doi: 10.1016/j.talanta.2024.126841. Epub 2024 Sep 10.
Paraquat (PQ) is a highly toxic herbicide that has been prohibited in almost 70 countries, but remains in use worldwide. Thus, routine on-site PQ monitoring is a key mechanism to ensure safety and efficiently enforce regulations. Herein, a label-free portable electrochemical aptasensor for the detection of PQ was developed by utilizing aptamer designed to specifically recognize PQ. The aptasensor employs square-wave voltammetry (SWV) to quantify PQ binding on the aptamer-functionalized electrode surface by tracking the downstream oxygen reduction reaction. It provided a detection range spanning from 0.01 to 100.0 μg mL PQ with a limit of detection (LOD) of 8.9 ng mL. Validation against spiked tap water, pomegranate juice, and orange juice revealed recovery rate performances of 75 %-130 %. The aptasensor demonstrates promising feasibility for PQ detection in real-world applications, offering remarkable portability and operational simplicity. Notably, it can operate without supplementary redox agents, requiring only sample incubation and subsequent washing steps.
百草枯（PQ）是一种剧毒除草剂，已被近 70 个国家禁止使用，但仍在全球范围内使用。因此，常规的现场 PQ 监测是确保安全和有效执行法规的关键机制。在这里，我们开发了一种无标签的便携式电化学适体传感器，用于检测 PQ，该传感器利用针对 PQ 特异性识别的适体。该适体传感器采用方波伏安法（SWV），通过跟踪下游的氧还原反应，定量检测在适体功能化电极表面上结合的 PQ。它提供了一个检测范围，从 0.01 到 100.0μg mL PQ，检测限（LOD）为 8.9ng mL。对加标自来水、石榴汁和橙汁的验证表明，回收率为 75%-130%。该适体传感器在实际应用中对 PQ 的检测具有良好的可行性，具有出色的便携性和操作简单性。值得注意的是，它可以在没有补充氧化还原试剂的情况下运行，只需要样品孵育和后续的清洗步骤。
