The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Talanta. 2025 Jan 1;281:126847. doi: 10.1016/j.talanta.2024.126847. Epub 2024 Sep 12.
Heparin, a widely studied glycosaminoglycan, plays crucial roles in the regulation of various physiological and pathological processes. Therefore, it's important to develop highly selective and sensitive methods for convenient monitoring of heparin levels in biological systems. We report the design and synthesis of FeO@PDA@MnO nanoparticles (FPM-NPs), which exhibit dual enzymatic activities, enabling quantitative detection of heparin. The FPM-NPs feature a unique tri-layer spherical shell structure, possessing both peroxidase-like and oxidase-like activities, and catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence or absence of HO. Remarkably, upon co-incubated with heparin, the oxidase activity of FPM-NPs decreases, while the peroxidase activity increases. By leveraging these dual enzymatic properties of FPM-NPs, a highly sensitive and specific colorimetric detection of heparin is achieved, with a detection limit reaching 6.51 nM and a good linear response to quantify heparin ranging 10-800 nM. Additionally, the developed FPM-NPs are successfully applied to measure heparin in fetal bovine serum samples. We also extend this detection method to a paper-based chip, enabling portable detection of heparin through grayscale analysis of mobile phone photographs. The multi-nanozyme-based heparin detection approach provides a new perspective for future research on expanding the application of nanocomposite materials in biomedical detection and analysis.
肝素是一种广泛研究的糖胺聚糖，在调节各种生理和病理过程中发挥着关键作用。因此，开发高度选择性和灵敏的方法来方便地监测生物系统中的肝素水平非常重要。我们报告了 FeO@PDA@MnO 纳米粒子（FPM-NPs）的设计和合成，其表现出双重酶活性，能够定量检测肝素。FPM-NPs 具有独特的三层球形壳结构，同时具有过氧化物酶和氧化酶样活性，并在存在或不存在 HO 的情况下催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化。值得注意的是，当与肝素共孵育时，FPM-NPs 的氧化酶活性降低，而过氧化物酶活性增加。利用 FPM-NPs 的这两种酶活性，可以实现肝素的高灵敏度和特异性比色检测，检测限达到 6.51 nM，对肝素的定量范围为 10-800 nM 具有良好的线性响应。此外，开发的 FPM-NPs 成功应用于测量胎牛血清样品中的肝素。我们还将这种检测方法扩展到纸质芯片上，通过手机照片的灰度分析实现肝素的便携式检测。基于多纳米酶的肝素检测方法为未来研究扩展纳米复合材料在生物医学检测和分析中的应用提供了新的视角。
