Wang J P, Hsu M F, Teng C M
Am J Chin Med. 1985;13(1-4):109-18. doi: 10.1142/S0192415X85000150.
The water extract of Hsien-Ho-T'sao (HHT) produced a dose-dependent inhibition on collagen-induced aggregation of platelet-rich plasma (PRP). The IC50 was about 3.5 mg/ml. In addition, HHT inhibited also the aggregation induced by ADP, A23187 or arachidonate in PRP. Greater inhibition was observed in the preparation of washed platelets. Increase of the calcium concentration in medium could not overcome the inhibitory effect of HHT. ATP release from platelets induced by collagen or A23187 was inhibited by HHT. In the presence of EDTA, ATP release caused by thrombin or A23187 was also inhibited by HHT. Malondialdehyde and thromboxane B2 formation was greatly inhibited by HHT in platelets challenged by collagen and thrombin. In arachidonate-stimulated platelets, thromboxane B2, but not malondialdehyde formation was inhibited. HHT showed more marked inhibition on aggregation in the presence of indomethacin, creatine phosphate/creatine phosphokinase or a combination of both. Hydrogen peroxide-induced hemolysis was marked reduced by HHT. It was concluded that HHT might have some membrane-active properties which interfered with the activation of phospholipase A2.
咸丰草水提取物（HHT）对胶原蛋白诱导的富含血小板血浆（PRP）聚集产生剂量依赖性抑制作用。半数抑制浓度（IC50）约为3.5毫克/毫升。此外，HHT还抑制PRP中由二磷酸腺苷（ADP）、A23187或花生四烯酸盐诱导的聚集。在洗涤血小板制剂中观察到更强的抑制作用。培养基中钙浓度的增加不能克服HHT的抑制作用。HHT抑制胶原蛋白或A23187诱导的血小板中三磷酸腺苷（ATP）释放。在存在乙二胺四乙酸（EDTA）的情况下，凝血酶或A23187引起的ATP释放也被HHT抑制。在胶原蛋白和凝血酶刺激的血小板中，HHT极大地抑制了丙二醛和血栓素B2的形成。在花生四烯酸盐刺激的血小板中，HHT抑制血栓素B2的形成，但不抑制丙二醛的形成。在存在吲哚美辛、磷酸肌酸/磷酸肌酸激酶或两者组合的情况下，HHT对聚集的抑制作用更明显。HHT显著降低了过氧化氢诱导的溶血。得出的结论是，HHT可能具有一些膜活性特性，可干扰磷脂酶A2的激活。
