University of Granada, Laboratory of Genetic Identification & Human Rights (LABIGEN-UGR), Department of Legal Medicine, Faculty of Medicine, Av. Investigación 11 - PTS - 18016 Granada, Spain.
University of Granada, Laboratory of Genetic Identification & Human Rights (LABIGEN-UGR), Department of Legal Medicine, Faculty of Medicine, Av. Investigación 11 - PTS - 18016 Granada, Spain.
Sci Justice. 2024 Sep;64(5):543-548. doi: 10.1016/j.scijus.2024.07.007. Epub 2024 Jul 31.
Y chromosome short tandem repeats (Y-STRs) typing is a useful tool in scenarios such as mass graves analysis or disaster victim identification and has become a routine analysis in many laboratories. Not many comparisons have been performed with the currently available commercial kits, much less with degraded skeletal remains. This research aims to evaluate the performance of three commercial Y-STR kits: Yfiler™ Plus, PowerPlex® Y23, and Investigator® Argus Y-28 in 63 degraded skeletal remains from mass graves. PowerPlex® Y23 yields more reportable markers and twice the RFU on average, while Yfiler™ Plus and Investigator® Argus Y-28 exhibited a similar behaviour. Additionally, Argus Y-28, which has not been tested with this kind of samples in literature before, showed a good performance. Finally, a predictive model was attempted to be developed from quantification and autosomal STR data. However, no acceptable model could be obtained. Nevertheless, good Y-STR typing results may be expected if at least 50 pg DNA input is used or 13 autosomal markers were previously obtained.
Y 染色体短串联重复序列（Y-STRs）分型是在大规模墓地分析或灾难遇难者识别等场景中非常有用的工具，并且已经成为许多实验室的常规分析。与目前可用的商业试剂盒相比，与降解骨骼遗骸相比，进行的比较并不多。本研究旨在评估三种商业 Y-STR 试剂盒：Yfiler™ Plus、PowerPlex® Y23 和 Investigator® Argus Y-28 在 63 例来自大规模墓地的降解骨骼遗骸中的性能。PowerPlex® Y23 产生更多可报告的标记物，平均 RFU 增加一倍，而 Yfiler™ Plus 和 Investigator® Argus Y-28 表现出相似的行为。此外，Argus Y-28 之前在文献中尚未对此类样本进行过测试，但其表现良好。最后，尝试从定量和常染色体 STR 数据开发预测模型。然而，无法获得可接受的模型。尽管如此，如果使用至少 50pg DNA 输入或之前获得 13 个常染色体标记，则可以预期良好的 Y-STR 分型结果。
