Ferreira-Silva Benedita, Fonseca-Cardoso Margarida, Porto Maria João, Magalhães Teresa, Cainé Laura
Department of Public Health, Forensic Sciences and Medical Education, Faculty of Medicine, University of Porto, Porto, Portugal.
National Institute of Legal Medicine and Forensic Sciences, Porto, Portugal.
J Forensic Sci. 2018 Nov;63(6):1836-1840. doi: 10.1111/1556-4029.13757. Epub 2018 Feb 21.
Biological evidence of sexual assault is one of the most difficult sample types to analyze in forensic laboratories. Y-STR markers are thus a valuable tool for analyzing these samples. The aim of this project was to compare three Y-STR commercial kits by analyzing their amplification performance on casework samples. Overall, 247 trace samples were analyzed with a Yfiler Plus PCR Amplification Kit (Applied Biosystems), PowerPlex Y23 (Promega ) System and AmpFLSTR Yfiler™ PCR Amplification Kit (Applied Biosystems). Comparing the amplification performance of the three kits, the first two were significantly more sensitive than the latter (p < 0.001). For samples, with a male DNA quantity less than 0.5 ng, the PowerPlex Y23 kit was the most sensitive and best performing kit, followed by the Yfiler Plus kit (p = 0.009). In conclusion, the Yfiler Plus and PowerPlex Y-23 kits are viable alternatives to older kits for samples with low amounts of male DNA.
性侵犯的生物学证据是法医实验室中最难分析的样本类型之一。因此，Y-STR标记是分析这些样本的宝贵工具。本项目的目的是通过分析三种Y-STR商业试剂盒在实际案件样本上的扩增性能来进行比较。总体而言，使用Yfiler Plus PCR扩增试剂盒（应用生物系统公司）、PowerPlex Y23（普洛麦格公司）系统和AmpFLSTR Yfiler™ PCR扩增试剂盒（应用生物系统公司）对247份痕量样本进行了分析。比较这三种试剂盒的扩增性能，前两种试剂盒比后一种试剂盒明显更灵敏（p < 0.001）。对于男性DNA量小于0.5 ng的样本，PowerPlex Y23试剂盒是最灵敏且性能最佳的试剂盒，其次是Yfiler Plus试剂盒（p = 0.009）。总之，对于男性DNA量低的样本，Yfiler Plus和PowerPlex Y-23试剂盒是比旧试剂盒更可行的替代品。
